Isolation and purification of phenylethanoid glycosides from Cistanche deserticola by high-speed counter-current chromatography

被引：79

作者：

Li, Li ^{[1
,2
]}

Tsao, Rong ^{[1
]}

Yang, Raymond ^{[1
]}

Liu, Chunming ^{[2
]}

Young, J. Christopher ^{[1
]}

Zhu, Honghui ^{[1
]}

机构：

[1] Agr & Agri Food Canada, Guelph Foos Res Ctr, Guelph, ON N1G 5C9, Canada

[2] Changchun Normal Univ, Dept Chem, Changchun 130032, Peoples R China

来源：

FOOD CHEMISTRY | 2008年 / 108卷 / 02期

关键词：

Cistanche deserticola; phenylethanoid; echinacoside; cistanoside A; acteoside; ioacteoside; 2'-acetylacteoside; HSCCC; LC-ESI-MS; NMR;

D O I：

10.1016/j.foodchem.2007.10.082

中图分类号：

O69 [应用化学];

学科分类号：

081704 [应用化学];

摘要：

Five phenylethanoid glycosides (PhGs), echinacoside, cistanoside A, acteoside, isoacteoside and 2'-acetylacteoside, were isolated and purified from Cistanche deserticola for the first time by high-speed counter-current chromatography (HSCCC) using two biphasic systems, one consisting of ethyl acetate-ethanol-water(5:0.5:4.5, v/v/v) and another of ethyl acetate-n-butanol-ethanol-water (0.5:0.5:0.1:1, v/v/v/v). A total of 28.5 mg of echinacoside, 18.4 mg of cistanoside A, 14.6 mg of acteoside, 30.1 mg of isoacteoside and 25.2 mg of 2'-acetylacteoside were purified from 1412 mg of the n-butanol extract of C deserticola, each at over 92.5% purity as determined by HPLC. The structures were identified by their retention time, UV, LC-ESI-MS in the negative ion mode, and confirmed by NMR experiments. The characteristic LC-ESI-MSn fragmentation pattern of the five compounds is discussed, and found to be a very specific and useful tool for the structural identification of PhGs from this important medicinal plant. Crown Copyright (C) 2007 Published by Elsevier Ltd. All rights reserved.

引用

页码：702 / 710

页数：9

共 24 条

[1]

Isolation and identification of four flavonoid constituents from the seeds of Oroxylum indicum by high-speed counter-current chromatography [J].