Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography

被引：101

作者：

Li, L

Tsao, R

Liu, ZQ

Liu, SY

Yang, R

Young, JC

Zhu, HH

Deng, ZY

Xie, MY

Fu, ZH

机构：

[1] Agr & Agri Food Canada, Food Res Program, Guelph, ON N1G 5C9, Canada

[2] Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, Changchun 130022, Peoples R China

[3] NanChang Univ, Minist Educ, Key Lab Food Sci, NanChang, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1063卷 / 1-2期

关键词：

Plantago psyllium; acteoside; isoacteoside; phenylethanoid glycoside; HSCCC; LC-APCI-MS; NMR;

D O I：

10.1016/j.chroma.2004.11.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1: 1, v:v). By injecting 200 mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165 mg of acteoside and 17.5 mg of isoacteoside from 978 mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：161 / 169

页数：9

共 27 条

[1] Isolation and identification of four flavonoid constituents from the seeds of Oroxylum indicum by high-speed counter-current chromatography [J].