Development of determination of di-n-octyl phthalate (DOP) residue by an indirect enzyme-linked immunosorbent assay

被引：37

作者：

Kuang, Hua ^{[1
]}

Xu, Liguang ^{[1
]}

Cui, Gang ^{[1
]}

Ma, Wei ^{[1
]}

Xu, Chuanlai ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2010年 / 21卷 / 03期

关键词：

phthalates; residues; hapten synthesis; ciELISA; polyvinyl chloride; immunoassay; resistance; SOLID-PHASE MICROEXTRACTION; COMPETITIVE FLUORESCENCE IMMUNOASSAY; PERFORMANCE LIQUID-CHROMATOGRAPHY; IONIZATION MASS-SPECTROMETRY; DISRUPTING CHEMICALS; INDUSTRIAL EFFLUENTS; LACTATIONAL EXPOSURE; GAS-CHROMATOGRAPHY; DIBUTYL PHTHALATE; MULTI-RESIDUES;

D O I：

10.1080/09540101003758962

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

In this research, the hapten di-n-octyl 4-aminophthalate (DOAP) was designed and synthesised successfully. It was used to couple with carrier proteins, bovine serum albumin (BSA) and ovalbumin (OVA) by diazotization reaction for immunogen (DOP-BSA) and coating antigen (DOP-OVA), respectively. Rabbits were immunised with DOP-BSA; polyclonal antiserum was raised and determined by competitive indirect enzyme-linked immunosorbent assay (ciELISA). After optimisation, a ciELISA was established. The quantitative working range for DOP was 5-75 ng/mL with the detection limit of 1.9 +/- 0.1 ng/mL and the IC50 of 19.2 +/- 1.1 ng/mL. The optimised ELISA had cross-reactivity of 22.6%, 17.6% and 21.2% with di-iso-octyl phthalate (DIOP), di-n-butyl phthalate (DBP) and di-hexyl phthalate (DHP), respectively. The result of the detection of polyvinyl chloride (PVC) samples showed that the immunoassay we developed had high-accuracy contrast with high-performance liquid chromatography electrospray ionisation tandem mass spectrometry analysis and it could be qualified to determine di-n-octyl phthalate (DOP) residue in PVC samples.

引用

页码：265 / 277

页数：13

共 35 条

[1]

Determination of endocrine disruptors in environmental water samples by stir bar sorptive extraction-liquid desorption -: Large volume injection-gas chromatography [J].