Biocompatibility of corroding tungsten coils: in vitro assessment of degradation kinetics and cytotoxicity on human cells

被引:87
作者
Peuster, M
Fink, C
von Schnakenburg, C
机构
[1] Ruhr Univ Bochum, Clin Congenital Heart Defects, Herz & Diabeteszentrum Nordrheim Westfalen, D-32545 Bad Oeynhausen, Germany
[2] Univ Hamburg, Dept Pediat Cardiol, Hamburg, Germany
[3] Univ Freiburg, Dept Pediat & Adolescent Med, Freiburg, Germany
关键词
biocompatibility; biodegradation; cell viability; corrosion; in vitro test; metal ion toxicity;
D O I
10.1016/S0142-9612(03)00274-6
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
This study was performed to determine the in vitro degradation rate of tungsten coils and to evaluate the potential local toxicity of tungsten on human pulmonary arterial endothelial (EC) and smooth muscle cells (SMC) and human dermal fibroblasts (FB). Therefore, tungsten coils were immersed in Ringer's solution and loss of mass and increase in tungsten concentration in the electrolyte were assessed in relation to immersion time (maximum: 140 days). Primary cultures of EC, SMC and FB were grown on multiplates for 1-10 days with ascending concentrations (0.1-5000 mug/ml) of tungsten in the growth medium. Metabolic activity was assessed by the use of the WST-1 Test (Roche). The in vitro degradation rate of the tungsten coil was 29 mug/day. EC were most susceptible to tungsten with a LD50 of 50mug/ml. In contrast, the LD50 for SMC was 100 and 1000 mug/ml for FB after 10 days of incubation. We conclude that, in vitro, degradation rate of tungsten coils is slow (29 mug/day). Very high (> 50 mug/ml [normal serum value 0.0002 mug/ml]) tungsten concentrations are needed to result in local cytopathologic effects on human EC, SMC and FB. These results correspond to clinical observations demonstrating the absence of toxicity of degrading tungsten coils in adult and pediatric patients despite elevated serum tungsten levels. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:4057 / 4061
页数:5
相关论文
共 12 条
[1]   Endovascular embolization of varicoceles: Resorption of tungsten coils in the spermatic vein [J].
Barrett, J ;
Wells, I ;
Riordan, R ;
Roobottom, C .
CARDIOVASCULAR AND INTERVENTIONAL RADIOLOGY, 2000, 23 (06) :457-459
[2]   In vivo degradation of tungsten embolisation coils [J].
Butler, TJ ;
Jackson, RW ;
Robson, JY ;
Owen, RJT ;
Delves, HT ;
Sieniawska, CE ;
Rose, JDG .
BRITISH JOURNAL OF RADIOLOGY, 2000, 73 (870) :601-603
[3]   An experimental study on thrombogenicity of various metallic microcoils with or without thrombogenic coatings [J].
Kim, TS ;
Park, JH ;
Lee, YS ;
Chung, JW ;
Han, MC .
INVESTIGATIVE RADIOLOGY, 1998, 33 (07) :407-410
[4]  
Leggett RW, 1997, SCI TOTAL ENVIRON, V206, P147
[5]   TRACE-ELEMENT REFERENCE VALUES IN TISSUES FROM INHABITANTS OF THE EUROPEAN COMMUNITY .1. A STUDY OF 46 ELEMENTS IN URINE, BLOOD AND SERUM OF ITALIAN SUBJECTS [J].
MINOIA, C ;
SABBIONI, E ;
APOSTOLI, P ;
PIETRA, R ;
POZZOLI, L ;
GALLORINI, M ;
NICOLAOU, G ;
ALESSIO, L ;
CAPODAGLIO, E .
SCIENCE OF THE TOTAL ENVIRONMENT, 1990, 95 :89-105
[6]   Dissolution of tungsten coils does not produce systemic toxicity, but leads to elevated levels of tungsten in the serum and recanalization of the previously occluded vessel [J].
Peuster, M ;
Fink, C ;
von Schnakenburg, C ;
Hausdorf, G .
CARDIOLOGY IN THE YOUNG, 2002, 12 (03) :229-235
[7]   Degradation of tungsten coils implanted into the subclavian artery of New Zealand white rabbits is not associated with local or systemic toxicity [J].
Peuster, M ;
Fink, C ;
Wohlsein, P ;
Bruegmann, M ;
Günther, A ;
Kaese, V ;
Niemeyer, M ;
Haferkamp, H ;
von Schnakenburg, C .
BIOMATERIALS, 2003, 24 (03) :393-399
[8]  
Peuster M, 2001, HEART, V85, P703
[9]   Different effects of rat interferon alpha, beta and gamma on rat hepatic stellate cell proliferation and activation [J].
Shen, H ;
Zhang, M ;
Minuk, GY ;
Gong, YW .
BMC CELL BIOLOGY, 2002, 3 (1)
[10]   Corrosion of tungsten spirals. A disturbing finding [J].
Weill, A ;
Ducros, V ;
Cognard, C ;
Piotin, M ;
Moret, J .
INTERVENTIONAL NEURORADIOLOGY, 1998, 4 (04) :337-340