Hydrogen peroxide activates the SoxRS regulon in vivo

被引：61

作者：

Manchado, M ^{[1
]}

Michán, C ^{[1
]}

Pueyo, C ^{[1
]}

机构：

[1] Univ Cordoba, Dept Bioquim & Biol Mol, E-14071 Cordoba, Spain

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 23期

关键词：

D O I：

10.1128/JB.182.23.6842-6844.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By multiplex reverse transcription-PCR, we demonstrate that the SoxRS response, which protects cells against superoxide toxicity, is triggered also by hydrogen peroxide. SoxR-dependent inductions of 7.3-, 7.6-, 4.6-, 2.2-, and 2.6-fold were quantified for soxS, micF, sodA, inaA, and fpr transcripts, respectively. This finding suggests an extensive and tight connectivity between different regulatory pathways in the Escherichia coli response to oxidative stress.

引用

页码：6842 / 6844

页数：3

共 17 条

[1] THE DPS PROMOTER IS ACTIVATED BY OXYR DURING GROWTH AND BY IHF AND A SIGMA(S) IN STATIONARY-PHASE [J].

ALTUVIA, S ;

ALMIRON, M ;

HUISMAN, G ;

KOLTER, R ;

STORZ, G .

MOLECULAR MICROBIOLOGY, 1994, 13 (02) :265-272

[2] In vivo transcription of nrdAB operon and of grxA and fpg genes is triggered in Escherichia coli lacking both thioredoxin and glutaredoxin 1 or thioredoxin and glutathione, respectively [J].

Gallardo-Madueño, R ;

Leal, JFM ;

Dorado, G ;

Holmgren, A ;

López-Barea, J ;

Pueyo, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (29) :18382-18388

[3] Activation of SoxR by overproduction of desulfoferrodoxin:: Multiple ways to induce the soxRS regulon [J].

Gaudu, P ;

Dubrac, S ;

Touati, D .

JOURNAL OF BACTERIOLOGY, 2000, 182 (06) :1761-1763

[4] A GLOBAL RESPONSE INDUCED IN ESCHERICHIA-COLI BY REDOX-CYCLING AGENTS OVERLAPS WITH THAT INDUCED BY PEROXIDE STRESS [J].

GREENBERG, JT ;

DEMPLE, B .

JOURNAL OF BACTERIOLOGY, 1989, 171 (07) :3933-3939

[5] Isolation of reductase for SoxR that governs an oxidative response regulon from Escherichia coli [J].

Kobayashi, K ;

Tagawa, S .

FEBS LETTERS, 1999, 451 (03) :227-230

[6] MUTATIONAL ANALYSIS OF THE REDOX-SENSITIVE TRANSCRIPTIONAL REGULATOR OXYR - REGIONS IMPORTANT FOR DNA-BINDING AND MULTIMERIZATION [J].

KULLIK, I ;

STEVENS, J ;

TOLEDANO, MB ;

STORZ, G .

JOURNAL OF BACTERIOLOGY, 1995, 177 (05) :1285-1291

[7]

Michán C, 1999, J BACTERIOL, V181, P2759

[8] 2-STAGE CONTROL OF AN OXIDATIVE STRESS REGULON - THE ESCHERICHIA-COLI SOXR PROTEIN TRIGGERS REDOX-INDUCIBLE EXPRESSION OF THE SOXS REGULATORY GENE [J].

NUNOSHIBA, T ;

HIDALGO, E ;

CUEVAS, CFA ;

DEMPLE, B .

JOURNAL OF BACTERIOLOGY, 1992, 174 (19) :6054-6060

[9] Transcriptional regulation of glutaredoxin and thioredoxin pathways and related enzymes in response to oxidative stress [J].

Prieto-Alamo, MJ ;

Jurado, J ;

Gallardo-Madueño, R ;

Monje-Casas, F ;

Holmgren, A ;

Pueyo, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (18) :13398-13405

[10] DUAL REGULATION OF INAA BY THE MULTIPLE ANTIBIOTIC-RESISTANCE (MAR) AND SUPEROXIDE (SOXRS) STRESS-RESPONSE SYSTEMS OF ESCHERICHIA-COLI [J].

ROSNER, JL ;

SLONCZEWSKI, JL .

JOURNAL OF BACTERIOLOGY, 1994, 176 (20) :6262-6269

← 1 2 →