Engineering of modular polyketide synthases to produce novel polyketides

被引：32

作者：

Carreras, CW

Santi, DV

机构：

[1] Kosan Biosci Inc, Burlingame, CA 94010 USA

[2] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1998年 / 9卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(98)80015-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyketides are important natural products produced by Actinomycetes and other organisms via the polymerization of coenzyme A-activated carboxylic acids. Modular polyketide synthases are large multifunctional enzymes that direct the biosynthetic process using a dedicated 'module' for each polymerization reaction, which specifies the unit to be polymerized, its oxidation state and stereochemistry. Over the past two years proof-of-principle has been demonstrated for technologies that modify or exchange modules to create hybrid enzymes that catalyze the biosynthesis of novel polyketides.

引用

页码：403 / 411

页数：9

共 48 条

[1] Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: Analysis of the enzymatic domains in the modular polyketide synthase [J].