Fabrication of novel chitosan nanofiber/gold nanoparticles composite towards improved performance for a cholesterol sensor

被引:47
作者
Gomathi, Palanisamy [1 ]
Ragupathy, Dhanusuraman [2 ]
Choi, Jin Hyun [3 ]
Yeum, Jeong Hyun [3 ]
Lee, Soo Chool [2 ]
Kim, Jae Chang [2 ]
Lee, Sang Hak [4 ]
Do Ghim, Han [1 ,3 ]
机构
[1] Kyungpook Natl Univ, Dept Text Syst Engn, Taegu 702701, South Korea
[2] Kyungpook Natl Univ, Dept Chem Engn, Taegu 702701, South Korea
[3] Kyungpook Natl Univ, Dept Adv Organ Mat Sci & Engn, Taegu 702701, South Korea
[4] Kyungpook Natl Univ, Dept Chem, Taegu 702701, South Korea
关键词
Chitosan; Biosensor; Gold nanoparticles; Cholesterol oxidase; CANDIDA-RUGOSA LIPASE; GOLD NANOPARTICLES; CARBON NANOTUBES; COVALENT IMMOBILIZATION; BIOSENSORS; OXIDASE; BIOCATALYSTS; MULTILAYER; OXIDATION; GELATIN;
D O I
10.1016/j.snb.2010.10.005
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel amperometric cholesterol biosensor was fabricated by the immobilization of ChOx (cholesterol oxidase) onto the chitosan nanofibers/gold nanoparticles (designated as CSNFs/AuNPs) composite network (NW). The fabrication involves preparation of chitosan nanofibers (CSNFs) and subsequent electrochemical loading of gold nanoparticles (AuNPs). Field emission scanning electron microscopy (FE-SEM) was used to investigate the morphology of CSNFs (sizes in the range of similar to 50-100 nm) and spherical AuNPs. Cyclic voltammetry, hydrodynamic voltammetry and amperometry were used to examine the performance of CSNF-AuNPs/ChOx biosensor. The CSNF-AuNPs/ChOx biosensor exhibited a wide linear response to cholesterol (concentration range of 1-45 mu M), good sensitivity (1.02 mu A/mu M). low response time (similar to 5 s) and excellent long term stability. The combined existence of AuNPs within CSNFs NW provides the excellent performance of the biosensor towards the electrochemical detection of cholesterol. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:44 / 49
页数:6
相关论文
共 28 条
  • [1] Synthesis and magnetic behavior of an array of nickel-filled carbon nanotubes
    Bao, JC
    Zhou, QF
    Hong, JM
    Xu, Z
    [J]. APPLIED PHYSICS LETTERS, 2002, 81 (24) : 4592 - 4594
  • [2] The National Cholesterol Education Program - Progress and prospects
    Cleeman, JI
    Lenfant, C
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1998, 280 (24): : 2099 - 2104
  • [3] Highly efficient biocatalysts via covalent immobilization of Candida rugosa lipase on ethylene glycol-modified gold-silica nanocomposites
    Drechsler, U
    Fischer, NO
    Frankamp, BL
    Rotello, VM
    [J]. ADVANCED MATERIALS, 2004, 16 (03) : 271 - +
  • [4] Activity of Candida rugosa lipase immobilized on γ-Fe2O3 magnetic nanoparticles
    Dyal, A
    Loos, K
    Noto, M
    Chang, SW
    Spagnoli, C
    Shafi, KVPM
    Ulman, A
    Cowman, M
    Gross, RA
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (07) : 1684 - 1685
  • [5] Development of a stable cholesterol biosensor based on multi-walled carbon nanotubes-gold nanoparticles composite covered with a layer of chitosan-room-temperature ionic liquid network
    Gopalan, Anantha Iyengar
    Lee, Kwang-Pill
    Ragupathy, Dhanusuraman
    [J]. BIOSENSORS & BIOELECTRONICS, 2009, 24 (07) : 2211 - 2217
  • [6] Chitosan mediated assembly of gold nanoparticles multilayer
    Huang, HZ
    Yang, XR
    [J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2003, 226 (1-3) : 77 - 86
  • [7] Enzyme-carrying polymeric nanofibers prepared via electrospinning for use as unique biocatalysts
    Jia, HF
    Zhu, GY
    Vugrinovich, B
    Kataphinan, W
    Reneker, DH
    Wang, P
    [J]. BIOTECHNOLOGY PROGRESS, 2002, 18 (05) : 1027 - 1032
  • [8] Optimization and characterization of dextran membranes prepared by electrospinning
    Jiang, HL
    Fang, DF
    Hsiao, BS
    Chu, B
    Chen, WL
    [J]. BIOMACROMOLECULES, 2004, 5 (02) : 326 - 333
  • [9] Chitin: a biomaterial in waiting
    Khor, E
    [J]. CURRENT OPINION IN SOLID STATE & MATERIALS SCIENCE, 2002, 6 (04) : 313 - 317
  • [10] FLOW-INJECTION ANALYSIS FOR TOTAL CHOLESTEROL WITH PHOTOMETRIC DETECTION
    KRUG, A
    GOBEL, R
    KELLNER, R
    [J]. ANALYTICA CHIMICA ACTA, 1994, 287 (1-2) : 59 - 64