Phosphorylation and activation of 13S condensin by Cdc2 in vitro

被引：249

作者：

Kimura, K ^{[1
]}

Hirano, M ^{[1
]}

Kobayashi, R ^{[1
]}

Hirano, T ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

来源：

SCIENCE | 1998年 / 282卷 / 5388期

关键词：

D O I：

10.1126/science.282.5388.487

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

13S condensin is a multisubunit protein complex essential for mitotic chromosome condensation in Xenopus egg extracts. Purified 135 condensin introduces positive supercoils into DNA in the presence of topoisomerase I and adenosine triphosphate in vitro. The supercoiling activity of 13S condensin was regulated by mitosis-specific phosphorylation. Immunodepletion, in vitro phospholylation, and peptide-mapping experiments indicated that Cdc2 is Likely to be the kinase that phosphorylates and activates 135 condensin. Multiple Cdc2 phosphorylation sites are clustered in the carboxyl-terminal domain of the XCAP-D2 (Xenopus chromosome-associated polypeptide D2) subunit. These results suggest that phosphorylation of 135 condensin by Cdc2 may trigger mitotic chromosome condensation in vitro.

引用

页码：487 / 490

页数：4

共 22 条

[1] Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with topoisomerase II [J].

Bhat, MA ;

Philp, AV ;

Glover, DM ;

Bellen, HJ .

CELL, 1996, 87 (06) :1103-1114

[2]

DAVIS FM, 1983, P NATL ACAD SCI-BIOL, V80, P2926, DOI 10.1073/pnas.80.10.2926

[3] CELL-CYCLE CONTROL OF HIGHER-ORDER CHROMATIN ASSEMBLY AROUND NAKED DNA INVITRO [J].

HIRANO, T ;

MITCHISON, TJ .

JOURNAL OF CELL BIOLOGY, 1991, 115 (06) :1479-1489

[4] SMC protein complexes and higher-order chromosome dynamics [J].

Hirano, T .

CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (03) :317-322

[5] Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein [J].

Hirano, T ;

Kobayashi, R ;

Hirano, M .

CELL, 1997, 89 (04) :511-521

[6] A HETERODIMERIC COILED-COIL PROTEIN REQUIRED FOR MITOTIC CHROMOSOME CONDENSATION IN-VITRO [J].

HIRANO, T ;

MITCHISON, TJ .

CELL, 1994, 79 (03) :449-458

[7] Chromosome dynamics: the SMC protein family [J].

Jessberger, R ;

Frei, C ;

Gasser, SM .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1998, 8 (02) :254-259

[8] ATP-dependent positive supercoiling of DNA by 13S condensin: A biochemical implication for chromosome condensation [J].

Kimura, K ;

Hirano, T .

CELL, 1997, 90 (04) :625-634

[9]

KIMURA K, UNPUB

[10] Mitotic chromosome condensation [J].

Koshland, D ;

Strunnikov, A .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1996, 12 :305-333

← 1 2 3 →