Long-term expansion of transplantable human fetal liver hematopoietic

被引:36
作者
Rollini, P
Kaiser, S
Hull, FV
Kapp, U
Leyvraz, S
机构
[1] Univ Lausanne Hosp, Ctr Pluridisciplinaire Oncol, Lausanne, Switzerland
[2] Univ Freiburg, Ctr Med, Dept Hematol Oncol, Freiburg, Germany
关键词
D O I
10.1182/blood-2003-06-1815
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Hematopoietic stem cells (HSCs), with their dual ability for self-renewal and multi-lineage differentiation, constitute an essential component of hematopoietic transplantations. Human fetal liver (FL) represents a promising alternative HSC source, and we previously reported simple culture conditions allowing long-term expansion of FL hematopoietic progenitors. In the present study, we used the nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mouse xenotransplantation assay to confirm that human FL is rich in NOD/SCID-repopulating cells (SRCs) and to show that these culture conditions repeatedly maintained short-and long-term SRCs from various FL samples for at least 28 days. Quantitative limited dilution analysis in NOD/SCID mice demonstrated for the first time that a 10-to over a 100-fold net expansion of FL SRCs could be achieved after 28 days of culture. The efficiency of this culture system may lead to an increase in the use of FL as a source of HSCs for transplantation in adult patients, as previously demonstrated with umbilical cord blood under different culture conditions.
引用
收藏
页码:1166 / 1170
页数:5
相关论文
共 28 条
  • [11] Ex vivo expansion of human adult stem cells capable of primary and secondary hemopoietic reconstitution
    Gammaitoni, L
    Bruno, S
    Sanavio, F
    Gunetti, M
    Kollet, O
    Cavalloni, G
    Falda, M
    Fagioli, F
    Lapidot, T
    Aglietta, M
    Piacibello, W
    [J]. EXPERIMENTAL HEMATOLOGY, 2003, 31 (03) : 261 - 270
  • [12] Mid-trimester fetal livers are a rich source of CD34+/++ cells for transplantation
    Golfier, F
    Bárcena, A
    Cruz, J
    Harrison, M
    Muench, MO
    [J]. BONE MARROW TRANSPLANTATION, 1999, 24 (05) : 451 - 461
  • [13] Unrelated donor hematopoietic cell transplantation: marrow or umbilical cord blood?
    Grewal, SS
    Barker, JN
    Davies, SM
    Wagner, JE
    [J]. BLOOD, 2003, 101 (11) : 4233 - 4244
  • [14] Distinct classes of human stem cells that differ in proliferative and self-renewal potential
    Guenechea, G
    Gan, OI
    Dorrell, C
    Dick, JE
    [J]. NATURE IMMUNOLOGY, 2001, 2 (01) : 75 - 82
  • [15] Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice
    Hogan, CJ
    Shpall, EJ
    Keller, G
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (01) : 413 - 418
  • [16] Functional differences between transplantable human hematopoietic stem cells from fetal liver, cord blood, and adult marrow
    Holyoake, TL
    Nicolini, FE
    Eaves, CJ
    [J]. EXPERIMENTAL HEMATOLOGY, 1999, 27 (09) : 1418 - 1427
  • [17] Huang S, 1998, EXP HEMATOL, V26, P1162
  • [18] ONTOGENY-RELATED CHANGES IN PROLIFERATIVE POTENTIAL OF HUMAN HEMATOPOIETIC-CELLS
    LANSDORP, PM
    DRAGOWSKA, W
    MAYANI, H
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 178 (03) : 787 - 791
  • [19] Unique differentiation programs of human fetal liver stem cells shown both in vitro and in vivo in NOD/SCID mice
    Nicolini, FE
    Holyoake, TL
    Cashman, JD
    Chu, PPY
    Lambie, K
    Eaves, CJ
    [J]. BLOOD, 1999, 94 (08) : 2686 - 2695
  • [20] Long-term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma-free cultures
    Peters, R
    Leyvraz, S
    Faes-van't Hull, E
    Jaunin, P
    Gerber, S
    Rollini, P
    [J]. BRITISH JOURNAL OF HAEMATOLOGY, 2002, 119 (03) : 792 - 802