Three new alkaloids from Veratrum grandiflorum Loes with inhibition activities on Hedgehog pathway

被引：20

作者：

Gao, Lijuan ^{[1
]}

Chen, Fengyang ^{[1
]}

Li, Xiaoyu ^{[1
]}

Xu, Shifang ^{[1
]}

Huang, Wenhai ^{[1
]}

Ye, Yiping ^{[1
]}

机构：

[1] Zhejiang Acad Med Sci, Inst Mat Med, Hangzhou 310013, Zhejiang, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2016年 / 26卷 / 19期

基金：

中国国家自然科学基金;

关键词：

Alkaloids; Veratrum grandiflorum Loes; Hedgehog pathway; Shh-LIGHT; 2; cells; Cancers; STEROIDAL ALKALOIDS; CANCER;

D O I：

10.1016/j.bmcl.2016.08.040

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Three new steroidal alkaloids 1-3, together with four known compounds 4-7, were isolated from the ethanol extract of Veratrum grandiflorum Loes. Their structures were elucidated by NMR (1D and 2D NMR) and MS spectroscopic data. The inhibition activities on Hedgehog (Hh) pathway were evaluated using a cell-based bioassay system (Shh-LIGHT 2 cells). The results showed that compounds 1-3 and 5 displayed inhibitory activities obviously with the IC50 values of 0.63-3.11 mu M. Among them, compound 5 showed the most prominent inhibition activity (IC50 = 0.63 +/- 0.02 mu M). Thus, these active alkaloids may be potent natural compounds as Hh pathway inhibitors for the treatment of various cancers. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：4735 / 4738

页数：4

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