Single-sided inkjet functionalization of silicon photonic microcantilevers

被引:5
作者
Ness, Stanley J. [1 ]
Kim, Seunghyun [2 ]
Woolley, Adam T. [3 ]
Nordin, Gregory P. [1 ]
机构
[1] Brigham Young Univ, Dept Elect & Comp Engn, Provo, UT 84602 USA
[2] Le Tourneau Univ, Sch Engn & Engn Technol, Longview, TX 75607 USA
[3] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 161卷 / 01期
关键词
Inkjet functionalization; Photonic microcantilever; Chemical vapor deposition; (3-Glycidoxypropyl) trimethoxysilane; Fluorescence microscopy; Biosensor; Microfluidics; BOVINE SERUM-ALBUMIN; SENSORS; TRANSDUCTION;
D O I
10.1016/j.snb.2011.09.026
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We investigate inkjet deposition of protein-containing fluid to individually functionalize top surfaces of silicon photonic microcantilevers (PMCLs) in a microcantilever array. An array is first prepared with vapor-deposited epoxysilane (3-glycidoxypropyl) trimethoxysilane (COPS), followed by inkjet deposition of functionalization fluid containing a model receptor molecule, biotinylated bovine serum albumin (bBSA). We find that a 61% (w/w) solution of glycerol achieves sufficient viscosity for reliable jetting, along with either Tween 20 or Triton X-100 as surfactant to prevent pooling of liquid on inkjet nozzles. We demonstrate that increasing relative humidity during incubation can be used to achieve uniform coverage of each PMCL surface with functionalization fluid through droplet swelling. Such swelling can also be designed to create a region off the base of each PMCL at which dissolved salts aggregate during droplet drying, thereby avoiding interference with or fouling of receptor molecule sites on PMCLs for significant improvement in active site density and uniformity. After functionalization, incubation, and droplet drying, a silicon PMCL die is integrated with polydimethylsiloxane (PDMS) microfluidics such that a PMCL array forms the bottom surface of a flow channel. A solution containing Alexa Fluor 514 labeled streptavidin is introduced into the flow channel to bind to bBSA on PMCLs. Fluorescence imaging is used to comparatively evaluate the uniformity and density of active receptor molecule attachment to the PMCLs for functionalization fluids with different pH and buffer species. In each case uniformity is enhanced by formation of a bulb at the microcantilever base during droplet swelling. (C) 2011 Published by Elsevier B.V.
引用
收藏
页码:80 / 87
页数:8
相关论文
共 35 条
  • [31] Folded cavity SOI microring sensors for high sensitivity and real time measurement of biomolecular binding
    Xu, D. -X.
    Densmore, A.
    Delage, A.
    Waldron, P.
    McKinnon, R.
    Janz, S.
    Lapointe, J.
    Lopinski, G.
    Mischki, T.
    Post, E.
    Cheben, P.
    Schmid, J. H.
    [J]. OPTICS EXPRESS, 2008, 16 (19): : 15137 - 15148
  • [32] Microcantilever (MCL) biosensing
    Yan, Xiaodong
    Ji, Hai-Feng
    Thundat, Thomas
    [J]. CURRENT ANALYTICAL CHEMISTRY, 2006, 2 (03) : 297 - 307
  • [33] Nerve agents detection using a Cu2+/L-cysteine bilayer-coated microcantilever
    Yang, YM
    Ji, HF
    Thundat, T
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (05) : 1124 - 1125
  • [34] ELISA microarray technology as a high-throughput system for cancer biomarker validation
    Zangar, Richard C.
    Daly, Don S.
    White, Amanda M.
    [J]. EXPERT REVIEW OF PROTEOMICS, 2006, 3 (01) : 37 - 44
  • [35] Multiwell micromechanical cantilever array reader for biotechnology
    Zhang, R.
    Best, A.
    Berger, R.
    Cherian, S.
    Lorenzoni, S.
    Macis, E.
    Raiteri, R.
    Cain, R.
    [J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 2007, 78 (08)