Defining the transcriptome and proteome in three functionally different human cell lines

被引：282

作者：

Lundberg, Emma ^{[1
]}

Fagerberg, Linn ^{[2
]}

Klevebring, Daniel ^{[2
]}

Matic, Ivan ^{[3
]}

Geiger, Tamar ^{[3
]}

Cox, Juergen ^{[3
]}

Algenas, Cajsa ^{[2
]}

Lundeberg, Joakim ^{[1
]}

Mann, Matthias ^{[3
]}

Uhlen, Mathias ^{[1
,2
]}

机构：

[1] Royal Inst Technol, Sci Life Lab, S-17165 Stockholm, Sweden

[2] Royal Inst Technol, AlbaNova Univ Ctr, Sch Biotechnol, S-17165 Stockholm, Sweden

[3] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

来源：

MOLECULAR SYSTEMS BIOLOGY | 2010年 / 6卷

关键词：

cell lines; expression; human; proteome; transcriptome; MESSENGER-RNA EXPRESSION; HUMAN GENOME; YEAST; QUANTIFICATION; IDENTIFICATION; FRACTIONATION; EFFICIENCY; SIGNATURES; ABUNDANCE; SEQUENCE;

D O I：

10.1038/msb.2010.106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An essential question in human biology is how cells and tissues differ in gene and protein expression and how these differences delineate specific biological function. Here, we have performed a global analysis of both mRNA and protein levels based on sequence-based transcriptome analysis (RNA-seq), SILAC-based mass spectrometry analysis and antibody-based confocal microscopy. The study was performed in three functionally different human cell lines and based on the global analysis, we estimated the fractions of mRNA and protein that are cell specific or expressed at similar/different levels in the cell lines. A highly ubiquitous RNA expression was found with > 60% of the gene products detected in all cells. The changes of mRNA and protein levels in the cell lines using SILAC and RNA ratios show high correlations, even though the genome-wide dynamic range is substantially higher for the proteins as compared with the transcripts. Large general differences in abundance for proteins from various functional classes are observed and, in general, the cell-type specific proteins are low abundant and highly enriched for cell-surface proteins. Thus, this study shows a path to characterize the transcriptome and proteome in human cells from different origins. Molecular Systems Biology 6: 450; published online 21 December 2010; doi:10.1038/msb.2010.106

引用

页数：9

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