Laser irradiated growth of protein crystal

被引：122

作者：

Adachi, H

Takano, K

Hosokawa, Y

Inoue, T

Mori, Y

Matsumura, H

Yoshimura, M

Tsunaka, Y

Morikawa, M

Kanaya, S

Masuhara, H

Kai, Y

Sasaki, T

机构：

[1] Osaka Univ, Dept Elect Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Dept Mat & Life Sci, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Dept Chem Mat, Suita, Osaka 5650871, Japan

[5] Osaka Univ, Venture Business Lab, Suita, Osaka 5650871, Japan

[6] JST, CREST, Suita, Osaka 5650871, Japan

[7] JST, PREST, Suita, Osaka 5650871, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS | 2003年 / 42卷 / 7B期

关键词：

protein crystal; nucleation; laser; short-pulse; supersaturated solution;

D O I：

10.1143/JJAP.42.L798

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We succeeded in the first ever generation of protein crystals by laser irradiation. We call this process Laser Irradiated Growth Technique (LIGHT). Effective crystallization was confirmed by applying an intense femtosecond laser. The crystallization period was dramatically shortened by LIGHT. In addition, protein crystals were obtained by LIGHT from normally uncrystallized conditions. These results indicate that intense femtosecond laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. The nuclei formation is possible primarily due to nonlinear nucleation processes of an intense femtosecond laser with a peak intensity of over a gigawatt (GW).

引用

收藏

页码：L798 / L800

页数：3

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