Ultra high-performance liquid chromatography of porphyrins

被引：6

作者：

Benton, Christopher M. ^{[1
,2
]}

Lim, Chang Kee ^{[2
,3
]}

Ritchie, Harald J. ^{[4
]}

Moniz, Caje ^{[2
]}

Jones, Donald J. L. ^{[1
]}

机构：

[1] Univ Leicester, RKCSB, Leicester LE2 7LX, Leics, England

[2] Kings Coll Hosp London, London SE5 9RS, England

[3] Univ London, Sch Biol Sci & Chem Sci, MRC Bioanalyt Sci Grp, London WC1E 7HX, England

[4] Thermo Fisher Sci, Runcorn, Cheshire, England

来源：

BIOMEDICAL CHROMATOGRAPHY | 2012年 / 26卷 / 03期

关键词：

UHPLC; porphyrins; porphyrias; porphyrin profiles in urine and faeces; TANDEM MASS-SPECTROMETRY; CUTANEA-TARDA; METABOLITES; PROFILES; URINE; FECES;

D O I：

10.1002/bmc.1662

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

An ultra high-performance liquid chromatographic (UHPLC) system was developed and optimized for the separation of porphyrins of clinical interest. Optimum conditions for the simultaneous separation of uroporphyrin, hepta-, hexa-, penta-carboxylic acid porphyrins and coproporphyrin and their type I and III isomers on a Thermo Hypersil BDS C18 column (2.4 mu m particle size, 100 x 2.1mm i.d.) using a gradient elution with 10% (v/v) acetonitrile in 1.0m ammonium acetate buffer (pH 5.16) and 10% (v/v) acetonitrile in methanol at a flow-rate of 0.4mL/min. The effect of mobile phase buffer molarity on the sensitivity of fluorescence detection and resolution of porphyrin isomers was investigated. The method was successfully applied to the analysis of porphyrins extracted from the urine and faeces of patients with various human porphyrias. Copyright (C) 2011 John Wiley & Sons, Ltd.

引用

页码：331 / 337

页数：7

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