Determination of oligosaccharide contents in 19 cultivars of chickpea (Cicer arietinum L) seeds by high performance liquid chromatography

被引：72

作者：

Xiang Xiaoli ^{[1
]}

Yang Liyi ^{[1
,2
]}

Hua Shuang ^{[1
]}

Li Wei ^{[1
]}

Sun Yi ^{[1
]}

Ma Hao ^{[3
]}

Zhang Jusong ^{[4
]}

Zeng Xiaoxiong ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China

[2] Univ Missouri, Coll Agr Food & Nat Resources, Div Food Syst & Bioengn, Columbia, MO 65211 USA

[3] Nanjing Agr Univ, Natl Ctr Soybean Improvement, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

[4] Xinjiang Agr Univ, Coll Agr, Xinjiang 830052, Peoples R China

来源：

FOOD CHEMISTRY | 2008年 / 111卷 / 01期

关键词：

chickpea (Cicer arietinum L) seed; alpha-galactooligosaccharide; sucrose; optimization; extraction method; HPLC;

D O I：

10.1016/j.foodchem.2008.03.039

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The extraction procedure of oligosaccharides from chickpea seeds was optimized and sufficient extraction was achieved with 50% of ethanol-water in a ratio of 10:1 for solvent to defatted chickpea meal at 50 degrees C for 30 min. Under the optimal extraction conditions, the contents of oligosaccharides in 19 cultivars of chickpea seeds were determined by HPLC. The results showed that the main alpha-galactooligosaccharides (alpha-GCIS) in chickpea seeds were raffinose, stachyose, verbascose and an unknown glycoside, which was isolated, purified, and identified as ciceritol. Ciceritol, the main sugar in all the chickpea samples, accounted for about 50% of the total alpha-GOS. There were considerable variations in the levels of alpha-GOS and sucrose between species, especially verbascose which could only be detected in 7 samples. With the highest amount of alpha-GOS and a low amount of sucrose, the 171 was the best choice for obtaining alpha-GOS for use as a prebiotic in functional foods. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：215 / 219

页数：5

共 25 条

[1]

Alles MS, 1999, AM J CLIN NUTR, V69, P980

[2] DETERMINATION, BY NMR-SPECTROSCOPY, OF THE STRUCTURE OF CICERITOL, A PSEUDOTRISACCHARIDE ISOLATED FROM LENTILS [J].

BERNABE, M ;

FENWICK, R ;

FRIAS, J ;

JIMENEZBARBERO, J ;

PRICE, K ;

VALVERDE, S ;

VIDALVALVERDE, C .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (06) :870-872

[3] Production, properties and applications of food-grade oligosaccharides [J].

Crittenden, RG ;

Playne, MJ .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1996, 7 (11) :353-361

[4] Optimization of extraction methods for determination of the raffinose family oligosaccharides in leguminous vine peas (Pisum sativum L.) and effects of blanching [J].

Ekvall, J. ;

Stegmark, R. ;

Nyman, M. .

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2007, 20 (01) :13-18

[5]

FAO, 1994, PROD YB

[6] IMPROVED METHODS OF OLIGOSACCHARIDE ANALYSIS FOR GENETIC-STUDIES OF LEGUME SEEDS [J].

FRIAS, J ;

HEDLEY, CL ;

PRICE, KR ;

FENWICK, GR ;

VIDALVALVERDE, C .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1994, 17 (11) :2469-2483

[7] DIETARY MODULATION OF THE HUMAN COLONIC MICROBIOTA - INTRODUCING THE CONCEPT OF PREBIOTICS [J].

GIBSON, GR ;

ROBERFROID, MB .

JOURNAL OF NUTRITION, 1995, 125 (06) :1401-1412

[8] Soluble carbohydrates in white lupin seeds matured at 13 and 28 degrees C [J].

Gorecki, RJ ;

Brenac, P ;

Clapham, WM ;

Willcott, JB ;

Obendorf, RL .

CROP SCIENCE, 1996, 36 (05) :1277-1282

[9]

HARPAL SS, 1984, J AGR FOOD CHEM, V32, P940

[10] Seed desiccation tolerance and storability: Dependence on flatulence-producing oligosaccharides and cyclitols-review and survey [J].

Horbowicz, Marcin ;

Obendorf, Ralph L. .

SEED SCIENCE RESEARCH, 1994, 4 (04) :385-405

← 1 2 3 →