Identification of a transcriptionally active hVH-5 pseudogene on 10q22-2

被引:7
作者
Berger, IR
Buschbeck, M
Bange, J
Ullrich, A
机构
[1] Max Planck Inst Biochem, Dept Biol Mol, D-82152 Martinsried, Germany
[2] Ctr Genom Regulat, Barcelona, Spain
[3] U3 Pharma AG, Martinsried, Germany
关键词
D O I
10.1016/j.cancergencyto.2004.10.010
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Mitogen-activated protein kinases (MAPKs) are important regulators of a vast number of biological functions that affect life and death of eukaryotic cells and are tightly regulated by the concerted action of several phosphatases. Among these is the human homologue of vaccinia virus HI phosphatase gene clone 5 (hVH-5) product, which dephosphorylates and thus inhibits members of the MAPK family. Here, we analyzed hVH-5 transcripts in mammary carcinoma cell lines and discovered a sequence with 88% similarity to hVH-5 transcripts. Because this variant of hVH-5 lacked intronic sequences in its genomic structure, we suggest it might be a processed pseudogene of hVH-5. psi hVH-5 transcripts were detected in human peripheral tissues as well as in 11 of 14 breast cancer cell lines. In respect to the normal hVH-5 gene, the pseudogene contains several point mutations and a frame shift due to the deletion of 2 bases that would lead to the truncation of the putative psi hVH-5 product. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:155 / 159
页数:5
相关论文
共 21 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]  
BRISTOW J, 1993, J BIOL CHEM, V268, P12919
[3]   Mammalian MAP kinase signalling cascades [J].
Chang, LF ;
Karin, M .
NATURE, 2001, 410 (6824) :37-40
[4]   Transcriptional analysis of the PTEN/MMAC1 pseudogene, ΨPTEN [J].
Fujii, GH ;
Morimoto, AM ;
Berson, AE ;
Bolen, JB .
ONCOGENE, 1999, 18 (09) :1765-1769
[5]   Genomic analysis of DUSP6, a dual specificity MAP kinase phosphatase, in pancreatic cancer [J].
Furukawa, T ;
Yatsuoka, T ;
Youssef, EM ;
Abe, T ;
Yokoyama, T ;
Fukushige, S ;
Soeda, E ;
Hoshi, M ;
Hayashi, Y ;
Sunamura, M ;
Kobari, M ;
Horii, A .
CYTOGENETICS AND CELL GENETICS, 1998, 82 (3-4) :156-159
[6]   CONFORMATION-SENSITIVE GEL-ELECTROPHORESIS FOR RAPID DETECTION OF SINGLE-BASE DIFFERENCES IN DOUBLE-STRANDED PCR PRODUCTS AND DNA FRAGMENTS - EVIDENCE FOR SOLVENT-INDUCED BENDS IN DNA HETERODUPLEXES [J].
GANGULY, A ;
ROCK, MJ ;
PROCKOP, DJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :10325-10329
[7]   Molecular fossils in the human genome: Identification and analysis of the pseudogenes in chromosomes 21 and 22 [J].
Harrison, PM ;
Hegyi, H ;
Balasubramanian, S ;
Luscombe, NM ;
Bertone, P ;
Echols, N ;
Johnson, T ;
Gerstein, M .
GENOME RESEARCH, 2002, 12 (02) :272-280
[8]   An expressed pseudogene regulates the messenger-RNA stability of its homologous coding gene [J].
Hirotsune, S ;
Yoshida, N ;
Chen, A ;
Garrett, L ;
Sugiyama, F ;
Takahashi, S ;
Yagami, K ;
Wynshaw-Boris, A ;
Yoshiki, A .
NATURE, 2003, 423 (6935) :91-96
[9]   Regulation of dual-specificity phosphatases M3/6 and hVH5 by phorbol esters - Analysis of a delta-like domain [J].
Johnson, TR ;
Biggs, JR ;
Winbourn, SE ;
Kraft, AS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (41) :31755-31762
[10]   Protein phosphatases and the regulation of mitogen-activated protein kinase signalling [J].
Keyse, SM .
CURRENT OPINION IN CELL BIOLOGY, 2000, 12 (02) :186-192