Miniaturization and parallelization of fluorescence immunoassays in nanotiter plates

被引：21

作者：

Seidel, M ^{[1
]}

Gauglitz, G ^{[1
]}

机构：

[1] Inst Phys & Theoret Chem, D-72076 Tubingen, Germany

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2003年 / 22卷 / 06期

关键词：

fluorescence immunoassay; fluorescence resonance energy transfer; ink-jet; nanotiter plate; phase separation; UItraHTS;

D O I：

10.1016/S0165-9936(03)00606-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Miniaturization and parallelization of fluorescence bioassays has gained ground in many fields of life sciences, pharmaceutical screening and chemical research and development. A heterogeneous fluorescence immunoassay for the detection of small analytes in nanoliter range using nanotiter plates (NTPs) is described. The phase-separation fluorescence immunoassay (PSFIA) is a competitive, heterogeneous immunoassay based on energy transfer to an immobilized acceptor dye at the surface of nanowells. (C) 2003 Published by Elsevier Science B.V.

引用

页码：385 / 394

页数：10

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