Violacein antimicrobial activity on Staphylococcus epidermidis and synergistic effect on commercially available antibiotics

被引：32

作者：

Dodou, H. V. ^{[1
]}

de Morais Batista, A. H. ^{[1
]}

Sales, G. W. P. ^{[1
]}

de Medeiros, S. C. ^{[1
]}

Rodrigues, M. L. ^{[1
]}

Nogueira, P. C. N. ^{[2
]}

Silveira, E. R. ^{[2
]}

Nogueira, N. A. P. ^{[1
]}

机构：

[1] Univ Fed Ceara, Dept Clin & Toxicol Anal, Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, Dept Organ Chem, Fortaleza, Ceara, Brazil

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2017年 / 123卷 / 04期

关键词：

antibiotic synergism; antibiotics; antimicrobial activity; Staphylococcus epidermidis; violacein; COMBINATION; AUREUS;

D O I：

10.1111/jam.13547

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

AimsThe study aimed to assess whether violacein has antimicrobial activity on Staphylococcus epidermidis and synergistically modulates the action of commercially available antimicrobial drugs. Methods and ResultsViolacein showed excellent antimicrobial activity on biofilm-forming and nonbiofilm-forming S. epidermidis strains (ATCC 35984) (ATCC 12228), with bacteriostatic (MIC=20gml(-1) and 10gml(-1) respectively) and bactericidal effects (MBC=20gml(-1) for both strains), observed in short periods of exposure. The violacein bactericidal concentration led to S. epidermidis death after 2-3h of exposure. Additionally, violacein synergistically modulated the activity of different antimicrobial classes on S. epidermidis ATCC 12228 (818%; n=9) and on S. epidermidis ATCC 35984 (545%; n=6), reducing the MIC of these antibiotics by up to 16-fold. ConclusionViolacein shows excellent antimicrobial activity on S. epidermidis strains. Significance and Impact of the StudyViolacein shows the potential for the development of a new drug for the treatment of infections caused by S. epidermidis.

引用

页码：853 / 860

页数：8

共 19 条

[1] A Commensal Strain of Staphylococcus epidermidis Overexpresses Membrane Proteins Associated with Pathogenesis When Grown in Biofilms [J].

Aguila-Arcos, S. ;

Ding, S. ;

Aloria, K. ;

Arizmendi, J. M. ;

Fearnley, I. M. ;

Walker, J. E. ;

Goni, F. M. ;

Alkorta, I. .

JOURNAL OF MEMBRANE BIOLOGY, 2015, 248 (03) :431-442

[2] RETRACTED: Synergistic combination of violacein and azoles that leads to enhanced killing of major human pathogenic dermatophytic fungi Trichophyton rubrum (Retracted article. See vol. 7, art no 496, 2017) [J].

Anju, S. ;

Kumar, Nishanth S. ;

Krishnakumar, B. ;

Kumar, B. S. Dileep .

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2015, 5

[3]

[Anonymous], 2012, APPROVED STANDARD

[4] Violet pigment production from liquid pineapple waste by Chromobacterium violaceum UTM5 and evaluation of its bioactivity [J].

Aruldass, Claira Arul ;

Rubiyatno ;

Venil, Chidambaram Kulandaisamy ;

Ahmad, Wan Azlina .

RSC ADVANCES, 2015, 5 (64) :51524-51536

[5] Linezolid resistance in clinical isolates of Staphylococcus epidermidis from German hospitals and characterization of two cfr-carrying plasmids [J].

Bender, Jennifer ;

Strommenger, Birgit ;

Steglich, Matthias ;

Zimmermann, Ortrud ;

Fenner, Ines ;

Lensing, Carmen ;

Dagwadordsch, Urantschimeg ;

Kekule, Alexander S. ;

Werner, Guido ;

Layer, Franziska .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2015, 70 (06) :1630-1638

[6] An overview of Staphylococcus epidermidis and Staphylococcus aureus with a focus on developing countries [J].

Chessa, Daniela ;

Ganau, Giulia ;

Mazzarello, Vittorio .

JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, 2015, 9 (06) :547-550

[7] Violacein: Properties and Production of a Versatile Bacterial Pigment [J].

Choi, Seong Yeol ;

Yoon, Kyoung-hye ;

Lee, Jin Il ;

Mitchell, Robert J. .

BIOMED RESEARCH INTERNATIONAL, 2015, 2015

[8]

Cleeland R, 1991, ANTIBIOTICS LAB MED

[9] Potential applications of violacein: a microbial pigment [J].

Duran, Marcela ;

Ponezi, Alexandre N. ;

Faljoni-Alario, Adelaide ;

Teixeira, Maria F. S. ;

Justo, Giselle Z. ;

Duran, Nelson .

MEDICINAL CHEMISTRY RESEARCH, 2012, 21 (07) :1524-1532

[10]

Jayaraman P, 2010, INT J BIOL SCI, V6, P556

← 1 2 →