The Drosophila Mi-2 Chromatin-Remodeling Factor Regulates Higher-Order Chromatin Structure and Cohesin Dynamics In Vivo

被引：29

作者：

Fasulo, Barbara ^{[1
]}

Deuring, Renate ^{[1
]}

Murawska, Magdalena ^{[2
]}

Gause, Maria ^{[3
]}

Dorighi, Kristel M. ^{[1
]}

Schaaf, Cheri A. ^{[3
]}

Dorsett, Dale ^{[3
]}

Brehm, Alexander ^{[2
]}

Tamkun, John W. ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA

[2] Univ Marburg, Inst Mol Biol & Tumor Res IMT, Marburg, Germany

[3] St Louis Univ, Sch Med, Edward A Doisy Dept Biochem & Mol Biol, St Louis, MO 63104 USA

来源：

PLOS GENETICS | 2012年 / 8卷 / 08期

基金：

美国国家卫生研究院;

关键词：

GENE-EXPRESSION; NIPPED-B; SMC PROTEINS; HISTONE H1; CHROMOSOME; COMPLEX; ISWI; HOMOLOG; TRANSCRIPTION; BINDING;

D O I：

10.1371/journal.pgen.1002878

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学];

摘要：

dMi-2 is a highly conserved ATP-dependent chromatin-remodeling factor that regulates transcription and cell fates by altering the structure or positioning of nucleosomes. Here we report an unanticipated role for dMi-2 in the regulation of higher-order chromatin structure in Drosophila. Loss of dMi-2 function causes salivary gland polytene chromosomes to lose their characteristic banding pattern and appear more condensed than normal. Conversely, increased expression of dMi-2 triggers decondensation of polytene chromosomes accompanied by a significant increase in nuclear volume; this effect is relatively rapid and is dependent on the ATPase activity of dMi-2. Live analysis revealed that dMi-2 disrupts interactions between the aligned chromatids of salivary gland polytene chromosomes. dMi-2 and the cohesin complex are enriched at sites of active transcription; fluorescence-recovery after photobleaching (FRAP) assays showed that dMi-2 decreases stable association of cohesin with polytene chromosomes. These findings demonstrate that dMi-2 is an important regulator of both chromosome condensation and cohesin binding in interphase cells.

引用

页数：14

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