Influence of poly(propylene sulfide-block-ethylene glycol) di-and triblock copolymer architecture on the formation of molecular adlayers on gold surfaces and their effect on protein resistance:: A candidate for surface modification in biosensor research

被引:61
作者
Feller, LM
Cerritelli, S
Textor, M
Hubbell, JA
Tosatti, SGP
机构
[1] ETH Honggerberg, Swiss Fed Inst Technol, Lab Surface Sci & Technol, BioInterface Grp, CH-8093 Zurich, Switzerland
[2] Ecole Polytech Fed Lausanne, Integrat Biosci Inst, Lausanne, Switzerland
[3] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, Lausanne, Switzerland
关键词
D O I
10.1021/ma051424m
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Control of nonspecific interactions between bioanalytical surfaces and proteins in the analyte is critical in the design of biosensor systems. Here we explore poly(propylene sulfide-block-ethylene glycol) (PPS-PEG) di- and triblock copolymer adlayers on gold to gain such control. Chemisorption of the PPS block permits a simple dip-and-rinse coating process. We synthesized different architectures of di- and triblock copolymers, varying the molecular weight of PEG between 1.1 and 5 kDa while keeping the PPS block constant at around 4 kDa, thus permitting systematic variations in ethylene glycol surface density in the adlayer. A simple dip-and-rinse process was used to produce PPS-PEG adlayers on gold substrates, which were characterized with surface plasmon resonance (SPR) and further confirmed by existing variable angle spectral ellipsometry (VASE), and X-ray photoelectron spectroscopy (XPS). Crowding in the PPS chemisorbed layer seemed to limit the polymer adsorption process. Subsequent exposure of PPS-PEG adlayers to protein adsorption (human serum albumin at 1 mg/mL or full-concentration human serum) was monitored with in situ SPR. Protein adsorption can be reduced up to 97% for human serum albumin and up to 96% for blood serum relative to bare gold substrates. Triblock copolymers were more effective than corresponding diblocks. The possibility to render gold surfaces bioinert is the basis for application in bioanalytical devices.
引用
收藏
页码:10503 / 10510
页数:8
相关论文
共 65 条
[51]   Air stability of alkanethiol self-assembled monolayers on silver and gold surfaces [J].
Schoenfisch, MH ;
Pemberton, JE .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (18) :4502-4513
[52]   INFLUENCE OF PREADSORBED BLOCK-COPOLYMERS ON PROTEIN ADSORPTION - SURFACE-PROPERTIES, LAYER THICKNESS, AND SURFACE COVERAGE [J].
SCHROEN, CGPH ;
STUART, MAC ;
MAARSCHALK, KV ;
VANDERPADT, A ;
VANTRIET, K .
LANGMUIR, 1995, 11 (08) :3068-3074
[53]   Poly(ethylene oxide) grafted to silicon surfaces: Grafting density and protein adsorption [J].
Sofia, SJ ;
Premnath, V ;
Merrill, EW .
MACROMOLECULES, 1998, 31 (15) :5059-5070
[54]   The effect of surface coverage and conformation of poly(ethylene oxide) (PEO) chains of poloxamer 407 on the biological fate of model colloidal drug carriers [J].
Stolnik, S ;
Daudali, B ;
Arien, A ;
Whetstone, J ;
Heald, CR ;
Garnett, MC ;
Davis, SS ;
Illum, L .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2001, 1514 (02) :261-279
[55]   SELF-ASSEMBLED MONOLAYERS ON GOLD GENERATED FROM ALKANETHIOLS WITH THE STRUCTURE RNHCOCH(2)SH [J].
TAMCHANG, SW ;
BIEBUYCK, HA ;
WHITESIDES, GM ;
JEON, N ;
NUZZO, RG .
LANGMUIR, 1995, 11 (11) :4371-4382
[56]   SURFACE MODIFICATION OF NANOPARTICLES BY PEO PPO BLOCK-COPOLYMERS TO MINIMIZE INTERACTIONS WITH BLOOD COMPONENTS AND PROLONG BLOOD-CIRCULATION IN RATS [J].
TAN, JS ;
BUTTERFIELD, DE ;
VOYCHECK, CL ;
CALDWELL, KD ;
LI, JT .
BIOMATERIALS, 1993, 14 (11) :823-833
[57]  
TANG CJ, UNPUB ANAL CHEM
[58]   STATIC SECONDARY ION MASS-SPECTROMETRY OF SELF-ASSEMBLED ALKANETHIOL MONOLAYERS ON GOLD [J].
TARLOV, MJ ;
NEWMAN, JG .
LANGMUIR, 1992, 8 (05) :1398-1405
[59]  
Tirelli N, 2002, J Biotechnol, V90, P3, DOI 10.1016/S1389-0352(01)00057-5
[60]   Peptide functionalized poly(L-lysine)-g-poly(ethylene glycol) on titanium:: resistance to protein adsorption in full heparinized human blood plasma [J].
Tosatti, S ;
De Paul, SM ;
Askendal, A ;
VandeVondele, S ;
Hubbell, JA ;
Tengvall, P ;
Textor, M .
BIOMATERIALS, 2003, 24 (27) :4949-4958