Cloning and biochemical characterization of a class A β-lactamase from Prevotella intermedia

被引：39

作者：

Madinier, I

Fosse, T

Giudicelli, J

Labia, R

机构：

[1] Hop Archet 2, Bacteriol Lab, F-06202 Nice 3, France

[2] Fac Chirurg Dentaire, Lab Pathobiol Orale, F-06357 Nice, France

[3] Fac Med, Biochim Lab, INSERM 145, F-06107 Nice 2, France

[4] CNRS, UMR 175, F-29000 Quimper, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2001年 / 45卷 / 08期

关键词：

D O I：

10.1128/AAC.45.8.2386-2389.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene encoding a B-lactamase of Prevotella intermedia was cloned and sequenced. This gene, called cfxA2, shared 98% identity with cfxA, the structural gene of a beta -lactamase previously described in Bacteroides vulgatus. The deduced protein sequence had a K272E substitution. CfxA2 had the characteristics of class A, group 2e beta -lactamases.

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页码：2386 / 2389

页数：4

相关论文

共 23 条

[1] Molecular characterization of the SHV-11 β-lactamase of Shigella dysenteriae [J].

Ahamed, J ;

Kundu, M .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (08) :2081-2083

[2] A STANDARD NUMBERING SCHEME FOR THE CLASS-A BETA-LACTAMASES [J].

AMBLER, RP ;

COULSON, AFW ;

FRERE, JM ;

GHUYSEN, JM ;

JORIS, B ;

FORSMAN, M ;

LEVESQUE, RC ;

TIRABY, G ;

WALEY, SG .

BIOCHEMICAL JOURNAL, 1991, 276 :269-270

[3] Antimicrobial susceptibilities of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens spp. isolated in Spain [J].

Andrés, MT ;

Chung, WO ;

Roberts, MC ;

Fierro, JF .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (11) :3022-3023

[4] BETA-LACTAMASE PRODUCTION AND SUSCEPTIBILITIES TO AMOXICILLIN, AMOXICILLIN-CLAVULANATE, TICARCILLIN, TICARCILLIN-CLAVULANATE, CEFOXITIN, IMIPENEM, AND METRONIDAZOLE OF 320 NON-BACTEROIDES-FRAGILIS-BACTEROIDES ISOLATES AND 129 FUSOBACTERIA FROM 28 UNITED-STATES CENTERS [J].

APPELBAUM, PC ;

SPANGLER, SK ;

JACOBS, MR .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (08) :1546-1550

[5] Characterization of beta-lactamase gene bla(PER-2,) which encodes an extended-spectrum class A beta-lactamase [J].

Bauernfeind, A ;

Stemplinger, I ;

Jungwirth, R ;

Mangold, P ;

Amann, S ;

Akalin, E ;

Ang, O ;

Bal, C ;

Casellas, JM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (03) :616-620

[6]

BOUTHERS AT, 1998, BIOCHEM J, V15, P1443

[7] A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].

BUSH, K ;

JACOBY, GA ;

MEDEIROS, AA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233

[8] Changes in beta-lactam antibiotic susceptibility and beta-lactamase production of clinical isolates of Bacteroides and Prevotella species over a 9 year period [J].

Edwards, R ;

Thirlwell, D ;

Greenwood, D .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1996, 37 (03) :636-638

[9] Prevalence of β-lactamase-producing strains among 149 anaerobic gram-negative rods isolated from periodontal pockets [J].

Fosse, T ;

Madinier, I ;

Hitzig, C ;

Charbit, Y .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 1999, 14 (06) :352-357

[10] Molecular evolution of the pathogenicity island of enterotoxigenic Bacteroides fragilis strains [J].

Franco, AA ;

Cheng, RK ;

Chung, GT ;

Wu, SG ;

Oh, HB ;

Sears, CL .

JOURNAL OF BACTERIOLOGY, 1999, 181 (21) :6623-6633