Comprehensive on-line LC/LC/MS of proteins

被引：294

作者：

Opiteck, GJ

Lewis, KC

Jorgenson, JW

Anderegg, RJ

机构：

[1] UNIV N CAROLINA, DEPT CHEM, CHAPEL HILL, NC 27599 USA

[2] GLAXO WELLCOME INC, DEPT ANALYT CHEM, RES TRIANGLE PK, NC 27709 USA

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 08期

关键词：

D O I：

10.1021/ac961155l

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This is a description of a comprehensive two-dimensional liquid chromatography (LC) system for the separation of protein mixtures. This system uses cation-exchange chromatography followed by reversed-phase chromatography(RPLC). The two LC systems are coupled by an eight-port valve equipped with two storage loops and under computer control. The RPLC effluent is sampled by both a UV detector and an electrospray mass spectrometer. In this way, complex mixtures of large biomolecules can be rapidly separated, desalted, and analyzed for molecular weight in less than 2 h. The system's utility is demonstrated with a mixture of standards and an Escherichia coli cell lysate.

引用

页码：1518 / 1524

页数：7