LanGT2 catalyzes the first glycosylation step during landomycin a biosynthesis

被引：43

作者：

Luzhetskyy, A

Taguchi, T

Fedoryshyn, M

Dürr, C

Wohlert, SE

Novikov, V

Bechthold, A

机构：

[1] Univ Freiburg, Inst Pharmazeut Wissensch, D-79104 Freiburg, Germany

[2] Natl Univ Lvivs Politech, Inst Chem & Chem Technol, UA-79000 Lvov, Ukraine

[3] Combinature Biopharm AG, D-13125 Berlin, Germany

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 08期

关键词：

biosynthesis; olivose; glycosylation; glycosyltransferases; landomycin;

D O I：

10.1002/cbic.200500018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glycosyltransferase LanGT2 is involved in the biosynthesis of the hexasacchoride side chain of the angucyclic antibiotic landomycin A. Its function was elucidated by targeted gene inactivation of lanGT2. The main metabolite of the obtained mutant was identified as tetrangulol (4), the progenitor of the landomycin aglycon (7). The lack of the sugar side chain indicates that LanGT2 catalyzes the priming glycosyl transfer in the hexasoccharide biosynthesis: the attachment Of a D-olivose to 0-8 of the polyketide backbone. Heterologous expression of urdGT2 from S. fradiae Tu2717 in this mutant resulted in the production of a novel C-glycosylated angucycline (6).

引用

页码：1406 / 1410

页数：5

共 19 条

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