Generation of novel landomycins M and O through targeted gene disruption

被引：39

作者：

Luzhetskyy, A

Zhu, LL

Gibson, M

Fedoryshyn, M

Dürr, C

Hofmann, C

Hoffmeister, D

Ostash, B

Mattingly, C

Adams, V

Fedorenko, V

Rohr, J

Bechthold, A

机构：

[1] Univ Freiburg, Inst Pharmazeut Wissensch, D-79104 Freiburg, Germany

[2] Univ Kentucky, Coll Pharm, Dept Pharmaceut Sci, Lexington, KY 40536 USA

[3] Lvov Natl Univ, Dept Genet & Biotechnol, UA-79005 Lvov, Ukraine

[4] Univ Kentucky, Coll Pharm, Dept Pharm Practice & Sci, Lexington, KY 40536 USA

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 04期

关键词：

antitumor agents; biosynthesis; glycosylation; hydroxylation; landomycin;

D O I：

10.1002/cbic.200400316

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two genes from Streptomyces cyanogenous S 136 that encode the reductose LonZ4 and the hydroxylose LonZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis. Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.

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收藏

页码：675 / 678

页数：4

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