The manipulation of single biomolecules

被引：77

作者：

Strick, T ^{[1
]}

Allemand, JFO ^{[1
]}

Croquette, V ^{[1
]}

Bensimon, D ^{[1
]}

机构：

[1] Ecole Normale Super, F-75231 Paris, France

来源：

PHYSICS TODAY | 2001年 / 54卷 / 10期

关键词：

D O I：

10.1063/1.1420553

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

The development of new tools for manipulating, visualizing, and studying single molecules and their interactions were discussed. The response of individual protein and deoxyribo nucleic acid (DNA) molecules were monitored to pulling and twisting. By this, biophysicists are expected to learn the size of molecular motors, their energy consumption, and rate-limiting transitions in enzymatic cycles. Cohesion forces and cooperative hydrogen bonding contributes to the stability of biomolecules and their native folded configurations. The study of DNA molecules, associated proteins and enzymes found magnetic-tweezer technique to be simple and powerful for analysis purposes.

引用

页码：46 / 51

页数：6

共 18 条

[1]

AJDARI A, 1997, REV MED PHYS, V69, P1269

[2]

Alberts B., 1994, MOL BIOL CELL

[3] Stretched and overwound DNA forms a Pauling-like structure with exposed bases [J].