Direct evidence for biased gene diversity estimates from dominant random amplified polymorphic DNA (RAPD) fingerprints

被引：93

作者：

Isabel, N ^{[1
]}

Beaulieu, J

Thériault, P

Bousquet, J

机构：

[1] Ctr Foresterie Laurentides, Serv Canadien Forets, Ressources Nat Canada, St Foy, PQ G1V 4C7, Canada

[2] Univ Laval, Ctr Rech Biol Forestiere, St Foy, PQ G1K 7P4, Canada

来源：

MOLECULAR ECOLOGY | 1999年 / 8卷 / 03期

关键词：

conifers; haploid megagametophytes; genetic diversity; genetic markers; RAPD phenotypes;

D O I：

10.1046/j.1365-294X.1999.00597.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relevance of using dominant random amplified polymorphic DNA (RAPD) fingerprints for estimating population differentiation was investigated when typically small population sample sizes were used. Haploid sexual tissues were first used to determine genotypes at RAPD loci for 75 eastern white pines (Pinus strobus L.) representing five populations. Dominant RAPD fingerprints were then inferred from genotypic data for each individual at each locus, and gene diversity estimates from both sources of data were compared. Genotypic information at RAPD loci indicated little or no differentiation among populations, similar to allozyme loci. However, estimates of population differentiation derived from dominant RAPD fingerprints according to various common methods of analysis were generally inflated, especially when all fragments were considered. Simulations showed that an increase in loci sampling and population sample sizes did not significantly alleviate the biases observed.

引用

页码：477 / 483

页数：7

共 23 条

[21] HOW SHOULD WE BOOTSTRAP ALLOZYME DATA [J].