Fiber-packed channel bioreactor for microfluidic protein digestion

被引：35

作者：

Fan, Huizhi ^{[1
]}

Chen, Gang ^{[1
]}

机构：

[1] Fudan Univ, Sch Pharm, Shanghai 200032, Peoples R China

来源：

PROTEOMICS | 2007年 / 7卷 / 19期

关键词：

fiberglass; microchip; microreactors; trypsin;

D O I：

10.1002/pmic.200700505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The fabrication and performance of a fiber-packed channel bioreactor in microchip along with its application in protein analysis were reported. The feasibility and performance of the unique microfluidic bioreactor were demonstrated by the tryptic digestion of myoglobin (MYO) and BSA. The on-chip digestion was carried out at a flow rate of 2.0 mu L/min and the digestion time was significantly reduced to less than 5 s. The digests were identified by MALDI-TOF MS with sequence coverages of 66% (MYO) and 40% (BSA) that were comparable to those obtained by the conventional in-solution tryptic digestion. The present fiber-based microchip bioreactor provides a promising platform for the high-throughput protein identification.

引用

页码：3445 / 3449

页数：5

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