Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids

被引:103
作者
Schwender, J [1 ]
Gemünden, C [1 ]
Lichtenthaler, HK [1 ]
机构
[1] Univ Karlsruhe, Inst Bot, Lehrstuhl 2, D-76128 Karlsruhe, Germany
关键词
charophyta; 1-deoxy-D-xylulose; green alga; isopentenyl diphosphate; isoprenoid biosynthesis; mevalonate;
D O I
10.1007/s004250000409
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The biosynthesis of the C(5) building block of isoprenoids, isopentenyl diphosphate (IPP), proceeds in higher plants via two basically different pathways: in the cytosolic compartment sterols are formed via mevalonate (MVA), whereas in the plastids the isoprenoids are formed via the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway (DOXP/MEP pathway). In the present investigation, we found for the Charophyceae, being close relatives to land plants, and in the original green flagellate Mesostigma viride the same IPP biosynthesis pattern as in higher plants: sterols are formed via MVA, and the phytol-moiety of chlorophylls via the DOXP/MEP pathway. In contrast, representatives of four classes of the Chlorophyta (Chlorophyceae, Ulvophyceae, Trebouxiophyceae, Prasinophyceae) did not incorporate MVA into sterols or phytol. Instead, they incorporated [1-(2)H(1)]-1-deoxy-D-xylulose into phytol and sterols. The results indicate that the entire Chlorophyta lineage, which is well separated from the land plant/Charophyceae lineage, is devoid of the acetate/MVA pathway and uses the DOXP/MEP pathway not only for plastidic, but also for cytosolic isoprenoid formation.
引用
收藏
页码:416 / 423
页数:8
相关论文
共 42 条
[21]   The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants [J].
Lichtenthaler, HK .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :47-65
[22]   Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate-independent pathway [J].
Lichtenthaler, HK ;
Schwender, J ;
Disch, A ;
Rohmer, M .
FEBS LETTERS, 1997, 400 (03) :271-274
[23]   Biosynthesis of terpenoids:: YchB protein of Escherichia coli phosphorylates the 2-hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [J].
Lüttgen, H ;
Rohdich, F ;
Herz, S ;
Wungsintaweekul, J ;
Hecht, S ;
Schuhr, CA ;
Fellermeier, M ;
Sagner, S ;
Zenk, MH ;
Bacher, A ;
Eisenreich, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (03) :1062-1067
[24]   Mesostigmatophyceae, a new class of streptophyte green algae revealed by SSU rRNA sequence comparisons [J].
Marin, B ;
Melkonian, M .
PROTIST, 1999, 150 (04) :399-417
[25]  
MOSS GP, 1989, EUR J BIOCHEM, V186, P429
[26]  
RAUSCHENBACH P, 1974, MESSUNG RADIOAKTIVEN
[27]   Cytidine 5′-triphosphate-dependent biosynthesis of isoprenoids:: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol [J].
Rohdich, F ;
Wungsintaweekul, J ;
Fellermeier, M ;
Sagner, S ;
Herz, S ;
Kis, K ;
Eisenreich, W ;
Bacher, A ;
Zenk, MH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (21) :11758-11763
[28]   Glyceraldehyde 3-phosphate and pyruvate as precursors of isoprenic units in an alternative non-mevalonate pathway for terpenoid biosynthesis [J].
Rohmer, M ;
Seemann, M ;
Horbach, S ;
BringerMeyer, S ;
Sahm, H .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (11) :2564-2566
[29]   ISOPRENOID BIOSYNTHESIS IN BACTERIA - A NOVEL PATHWAY FOR THE EARLY STEPS LEADING TO ISOPENTENYL DIPHOSPHATE [J].
ROHMER, M ;
KNANI, M ;
SIMONIN, P ;
SUTTER, B ;
SAHM, H .
BIOCHEMICAL JOURNAL, 1993, 295 :517-524
[30]   SAG - SAMMLUNG-VON-ALGENKULTUREN AT THE UNIVERSITY-OF-GOTTINGEN - CATALOG OF STRAINS 1994 [J].
SCHLOSSER, UG .
BOTANICA ACTA, 1994, 107 (03) :113-186