Application of phenotypic microarrays to environmental microbiology

被引：32

作者：

Borglin, Sharon ^{[1
]}

Joyner, Dominique ^{[1
]}

DeAngelis, Kristen M. ^{[1
,2
,3
]}

Khudyakov, Jane ^{[3
]}

D'haeseleer, Patrik ^{[3
,4
]}

Joachimiak, Marcin P. ^{[5
,6
]}

Hazen, Terry ^{[1
,3
,7
,8
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Dept Ecol, Berkeley, CA 94720 USA

[2] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA

[3] Joint Bioenergy Inst, Microbial Communities Grp, Emeryville, CA USA

[4] Lawrence Livermore Natl Lab, Biosci & Biotechnol Div, Livermore, CA USA

[5] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[6] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Virtual Inst Microbial Stress & Survival, Berkeley, CA 94720 USA

[7] Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA

[8] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2012年 / 23卷 / 01期

关键词：

DESULFOVIBRIO-VULGARIS HILDENBOROUGH; GROWTH CURVE; BACTERIA; PROFILES; STRESS; REDUCTION; MUTANTS; BIOLOGY; YEAST; TOOL;

D O I：

10.1016/j.copbio.2011.12.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Environmental organisms are extremely diverse and only a small fraction has been successfully cultured in the laboratory. Culture in micro wells provides a method for rapid screening of a wide variety of growth conditions and commercially available plates contain a large number of substrates, nutrient sources, and inhibitors, which can provide an assessment of the phenotype of an organism. This review describes applications of phenotype arrays to anaerobic and thermophilic microorganisms, use of the plates in stress response studies, in development of culture media for newly discovered strains, and for assessment of phenotype of environmental communities. Also discussed are considerations and challenges in data interpretation and visualization, including data normalization, statistics, and curve fitting.

引用

页码：41 / 48

页数：8

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