Metalloprotein design

被引:39
作者
Hellinga, HW [1 ]
机构
[1] DUKE UNIV, MED CTR, DEPT BIOCHEM, DURHAM, NC 27710 USA
关键词
D O I
10.1016/S0958-1669(96)80121-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The rational design of novel proteins offers a new method of studying structure and function, and makes possible the construction of new biomaterials. The richness of metal chemistry, the relative ease of creating stable complexes, and the remarkable degree of subtle, highly specific control of reactivity imposed by the protein matrix upon the metal center make metalloprotein design a very fruitful area for the exploration and application of design techniques. So far, most designs have concentrated on the exploration of simple metal-chelation properties. Even so, this has led to the development of new methods for protein stabilization and affinity purification, of metal biosensors, of novel strategies for control of protein activity, and of model systems for the exploration of fundamental principles of molecular recognition.
引用
收藏
页码:437 / 441
页数:5
相关论文
共 56 条
  • [31] ENTROPIC STABILIZATION OF A MUTANT HUMAN LYSOZYME INDUCED BY CALCIUM-BINDING
    KUROKI, R
    KAWAKITA, S
    NAKAMURA, H
    YUTANI, K
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) : 6803 - 6807
  • [32] Lippard S.J., 1994, PRINCIPLES BIOINORGA
  • [33] RACK-INDUCED BONDING IN BLUE-COPPER PROTEINS
    MALMSTROM, BG
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 223 (03): : 711 - 718
  • [34] STRUCTURE OF AN ENGINEERED, METAL-ACTUATED SWITCH IN TRYPSIN
    MCGRATH, ME
    HAYMORE, BL
    SUMMERS, NL
    CRAIK, CS
    FLETTERICK, RJ
    [J]. BIOCHEMISTRY, 1993, 32 (08) : 1914 - 1919
  • [35] MCPHALEN CA, 1991, ADV PROTEIN CHEM, V42, P77
  • [36] RUTHENIUM-MEDIATED PROTEIN CROSS-LINKING AND STABILIZATION
    MUHEIM, A
    TODD, RJ
    CASIMIRO, DR
    GRAY, HB
    ARNOLD, FH
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (12) : 5312 - 5313
  • [37] MULLER HN, 1994, BIOCHEMISTRY-US, V33, P14126
  • [38] THE DESIGN OF METAL-BINDING SITES IN PROTEINS
    REGAN, L
    [J]. ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1993, 22 : 257 - 281
  • [39] A TETRAHEDRAL ZINC(II)-BINDING SITE INTRODUCED INTO A DESIGNED PROTEIN
    REGAN, L
    CLARKE, ND
    [J]. BIOCHEMISTRY, 1990, 29 (49) : 10878 - 10883
  • [40] PROTEIN DESIGN - NOVEL METAL-BINDING SITES
    REGAN, L
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (07) : 280 - 285