Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A

被引：54

作者：

Luzhetskyy, A

Fedoryshyn, M

Dürr, C

Taguchi, T

Novikov, V

Bechthold, A

机构：

[1] Univ Freiburg, Inst Pharmazeut Wissensch, D-79104 Freiburg, Germany

[2] Natl Univ, Inst Chem & Chem Technol, UA-89000 Lvov, Ukraine

来源：

CHEMISTRY & BIOLOGY | 2005年 / 12卷 / 07期

关键词：

D O I：

10.1016/j.chembiol.2005.05.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Detailed studies on the biosynthesis of the hexasaccharide side chain of landomycin A, produced by S. cyanogenus S136, revealed the function of each glycosyltransferase gene of the biosynthetic gene cluster. Analyses of generated mutants as well as feeding experiments allowed us to determine that LanGT2 and LanGT3 catalyze the attachment of one sugar, whereas LanGT1 and LanGT4 attach two sugars during landomycin A biosynthesis. The generation of a IanZ2 deletion mutant provided evidence that LanZ2 is controlling the elongation of the saccharide side chain.

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收藏

页码：725 / 729

页数：5

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