Immunoassay of prostate-specific antigen (PSA) using resonant frequency shift of piezoelectric nanomechanical microcantilever

被引:163
作者
Lee, JH
Hwang, KS
Park, J
Yoon, KH
Yoon, DS
Kim, TS [1 ]
机构
[1] KIST, Microsyst Res Ctr, Seoul 136791, South Korea
[2] Yonsei Univ, Dept Ceram Engn, Seoul 120749, South Korea
关键词
nanomechanics; PSA; piezoelectric; PZT cantilever; quantitative analysis; protein mass detection;
D O I
10.1016/j.bios.2004.09.024
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We designed and fabricated the nanomechanical Pb(Zr0.52Ti0.48)O-3 (PZT) cantilever; we demonstrated a novel electrical measurement, under a controlled ambient temperature and humidity, for label-free detection of a prostate-specific antigen (PSA); and we achieved a detection sensitivity as low as 10 pg/ml. For the fabrication of our nanomechanical PZT cantilevers, we used composite layers of Ta/Pt/PZT/Pt/SiO2 On a SiNx supporting layer for electrical self-sensing without external oscillators. This method allows PSA proteins to be detected via a simple electrical measurement of the resonant frequency change generated by the molecular interaction of the antigen (Ag) and the antibody (Ab). The resonant frequency shifted due to the specific binding of the PSA Ag to its Ab which is immobilized via calixcrown self-assembled monolayers on an Au surface deposited on a nanomechanical PZT cantilever. We determined the resonant frequency shift as the value of -172 Hz and -273 Hz, when the concentration of PSA Ag was 1 ng/ml, with the cantilever dimensions of 100 mu m x 300 mu m and 50 mu m x 150 mu m, respectively. Theoretical and experimental analysis suggests that the minimum detectable sensitivity for a resonant frequency shift due to a PSA Ag-Ab interaction depends on the dimensions of the nanomechanical PZT cantilever. These results also demonstrate that the experimentally measured resonant frequency shift is larger than that calculated theoretically due to the compressive stress of the PSA Ag-Ab interaction. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:2157 / 2162
页数:6
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