Isolation and characterization of peptide antibiotics LI-F04 and polymyxin B6 produced by Paenibacillus polymyxa strain JS']JSa-9

被引：38

作者：

Deng, Yang ^{[1
]}

Lu, Zhaoxin ^{[1
]}

Bi, Hua ^{[1
]}

Lu, Fengxia ^{[1
]}

Zhang, Chong ^{[1
]}

Bie, Xiaomei ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, Key Lab Food Proc & Qual Control, Minist Agr China, Nanjing 210095, Peoples R China

来源：

PEPTIDES | 2011年 / 32卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Paenibacillus polymyxa; LI-F04; Polymyxin B-6; Identification; Antimicrobial activity; BACILLUS-POLYMYXA; STRUCTURE ELUCIDATION; CYCLIC DEPSIPEPTIDES; SODIUM; KT-8;

D O I：

10.1016/j.peptides.2011.08.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Paenibacillus polymyxa JSa-9 had been found to produce five cyclic LI-F type antibiotics which were released into culture medium in accordance with our previous report. In this study, another three kinds of antagonistic compounds were extracted from P. polymyxa JSa-9 cell pellets and (or) spores by methanol. Using high performance liquid chromatography (HPLC) method, two antagonistic fractions were separated and collected from the methanol extract. One showed inhibition against Escherichia coli and Staphylococcus aureus, while the other was active against Aspergillus niger and S. aureus. By means of electrospray ionization mass spectroscopy (ESI-MS), infrared spectroscopy (IR), and amino acid analysis, two kinds of compounds from fraction B with molecular masses of 901 and 915 Da were characterized as the linear lipopeptide analogs of antibiotics LI-F04a and LI-F04b, respectively. Another antimicrobial substance from fraction A could be attributed to polymyxin B-6. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：1917 / 1923

页数：7

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