Rapid extraction of DNA from Escherichia coli and Cryptosporidium parvum for use in PCR

被引：20

作者：

Higgins, JA ^{[1
]}

Jenkins, MC ^{[1
]}

Shelton, DR ^{[1
]}

Fayer, R ^{[1
]}

Karns, JS ^{[1
]}

机构：

[1] USDA ARS, Beltsville, MD 20705 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 11期

关键词：

D O I：

10.1128/AEM.67.11.5321-5324.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All ability methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.

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收藏

页码：5321 / 5324

页数：4

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