Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow

被引:351
作者
De Ugarte, DA
Alfonso, Z
Zuk, PA
Elbarbary, A
Zhu, M
Ashjian, P
Benhaim, P
Hedrick, MH
Fraser, JK
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Med, Div Hematol & Oncol, Los Angeles, CA 90095 USA
[2] Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Sch Med, Dept Surg, Regenerat Bioengn & Repair Lab, Los Angeles, CA USA
[4] Univ Calif Los Angeles, Sch Med, Dept Orthopaed, Regenerat Bioengn & Repair Lab, Los Angeles, CA USA
关键词
mesenchymal stem cell; processed lipoaspirate cell; adipose; phenotype;
D O I
10.1016/S0165-2478(03)00108-1
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Our laboratory has characterized a population of stromal cells obtained from adipose tissue termed processed lipoaspirate cells (PLAs). PLAs, like bone-marrow derived mesenchymal stem cells (BM-MSCs), have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages, In order to better characterize these two multi-lineage populations, we examined the surface phenotype of both bone marrow and adipose tissue-derived cells from five patients undergoing surgery. PLA and BM-MSC cells were isolated, subcultivated, and evaluated for cell surface marker expression using flow cytometry. PLA and BM-MSC cells both expressed CD13, CD29, CD44, CD90, CD105, SH-3, and STRO-1. Differences in expression were noted for cell adhesion molecules CD49d (Integrin alphaA), CD54 (ICAM-1), CD34, and CD106 (VCAM-1). While markedly similar, the surface phenotypes of PLA and BM-MSC cells are distinct for several cell adhesion molecules implicated in hematopoietic stem cell homing, mobilization, and proliferation. (C) 2003 Published by Elsevier B.V.
引用
收藏
页码:267 / 270
页数:4
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