Protein identification using 2D-LC-MS/MS

被引：142

作者：

Delahunty, C ^{[1
]}

Yates, JR ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

METHODS | 2005年 / 35卷 / 03期

关键词：

proteomics; multidimensional chromatography; mass spectrometry; protein mixture analysis;

D O I：

10.1016/j.ymeth.2004.08.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multidimensional liquid chromatography techniques have been coupled to tandem mass spectrometry to provide a robust method to identify proteins in complex mixtures. Data acquisition is interfaced directly with search algorithms for identification through cross-correlation with databases. This review describes the most recent advances in methodologies for protein identification by mass spectrometry and describes the limitations of the application of the technologies. (c) 2004 Elsevier Inc. All rights reserved.

引用

页码：248 / 255

页数：8

共 31 条

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