Quantitative Label-Free Animal Sperm Imaging by Means of Digital Holographic Microscopy

被引：51

作者：

Di Caprio, Giuseppe ^{[1
]}

Gioffre, Mariano A. ^{[1
]}

Saffioti, Nunzia ^{[1
]}

Grilli, Simonetta ^{[2
]}

Ferraro, Pietro ^{[2
]}

Puglisi, Roberto ^{[3
]}

Balduzzi, Donatella ^{[3
]}

Galli, Andrea ^{[3
]}

Coppola, Giuseppe ^{[1
]}

机构：

[1] CNR, Ist Microelettron & Microsistemi, I-80131 Naples, Italy

[2] Ist Nazl Ott Applicata, I-80078 Pozzuoli, Italy

[3] Ist Sperimentale Italiano LazzaroSpallanzani, I-26027 Localita La Quercia, Rivolta Dadda, Italy

来源：

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS | 2010年 / 16卷 / 04期

关键词：

Digital holography microscope (DHM); in vivo cell analysis; label-free analysis; microfluidic; quantitative phase-contrast map; zootechny; PHASE-CONTRAST MICROSCOPY; CELL MORPHOMETRY; REFRACTIVE-INDEX; LIVING CELLS; RECONSTRUCTION; COMPENSATION;

D O I：

10.1109/JSTQE.2009.2036741

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A digital holographic microscope (DHM) has been employed in the retrieval and analysis of morphological images of bovine's sperm cells. Digital holography is a noncontact technique capable of investigating the shape of the sample without altering its characteristics and has been used for the first time in retrieving quantitative morphological information of sperm cells. Different spermatozoa have been analyzed by means of this technique allowing us to obtain 3-D images with precise topographical details and valuable information about morphological defects, provided with biological considerations. Moreover, by making use of a microfluidic system, the digital holographic technique has been employed to analyze unstained spermatozoa in their natural physiological surroundings. Detailed information on morphological images of spermatozoa acquired by DHM is expected to provide a better understanding of various reproductive pathways, which, in turn, can help in improving infertility management. This could constitute the basis of an alternative method for the zoothecnic industry aimed at the investigation of morphological features and the sorting of the motile sperm cells.

引用

页码：833 / 840

页数：8

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