Single-enzyme nanoparticles armored by a nanometer-scale organic/inorganic network

被引：209

作者：

Kim, J ^{[1
]}

Grate, JW ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Richland, WA 99352 USA

来源：

NANO LETTERS | 2003年 / 3卷 / 09期

关键词：

D O I：

10.1021/nl034404b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have developed armored single-enzyme nanoparticles (SENs) that surround each enzyme molecule with a porous composite organic/inorganic network of less than a few nanometers; thick. This approach has significantly stabilized two proteases (alpha-chymotrypsin, CT, and trypsin, TR), and the armor network around CT is sufficiently thin and porous that it does not place a large mass-transfer limitation on the substrate. These new hybrid enzyme nanostructures offer great potential as a method to stabilize enzymes for various applications.

引用

页码：1219 / 1222

页数：4

共 19 条

[1]

Blanch HW., 1997, BIOCH ENG, P163

[2] The search for the ideal biocatalyst [J].