Colistin-functionalised CdSe/ZnS quantum dots as fluorescent probe for the rapid detection of Escherichia coli

被引：65

作者：

Carrillo-Carrion, Carolina ^{[1
]}

Simonet, Bartolome M. ^{[1
]}

Valcarcel, Miguel ^{[1
]}

机构：

[1] Univ Cordoba, Dept Analyt Chem, E-14071 Cordoba, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 11期

关键词：

CdSe/ZnS Quantum dots (QDs); Colistin; Gram-negative bacteria; E. coli detection; GRAM-NEGATIVE BACTERIA; POLYMYXIN-B; SALMONELLA-TYPHIMURIUM; O157-H7; CDTE; NANOCRYSTALS; IMMUNOASSAY; RECOGNITION; SCATTERING; BIOSENSOR;

D O I：

10.1016/j.bios.2011.04.050

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Intensely fluorescent, colistin-functionalised CdSe/ZnS QDs (Colis-QDs) nanoparticles, are synthesized and used as sensitive probes for the detection of Escherichia coli, a Gram-negative bacteria. Colistin molecules are attached to the terminal carboxyl of the mercaptoacetic acid-capped QDs in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as amide bond promoters. The TEM analysis of bacteria treated with Colis-QDs conjugates showed the accumulation of Colis-QDs in the cell wall of E. coli. Under the recommended working conditions, the method provides a detection limit as few as 28 E. coli cells per mL, which is competitive which more elaborate detection systems. The simplicity of the method together with short analysis time (<15 min, without including preparation and photoactivation of the Colis-QDs conjugate) make the proposed approach useful as quick bacteria screening system. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：4368 / 4374

页数：7

共 42 条

[21] Fabrication of a disposable biosensor for Escherichia coli O157:H7 detection [J].

Muhammad-Tahir, Z ;

Alocilja, EC .

IEEE SENSORS JOURNAL, 2003, 3 (04) :345-351

[22] Bacterial detection using carbohydrate-functionalised CdS quantum dots: a model study exploiting E. coli recognition of mannosides [J].

Mukhopadhyay, Balaram ;

Martins, Maristela B. ;

Karamanska, Rositsa ;

Russell, David A. ;

Field, Robert A. .

TETRAHEDRON LETTERS, 2009, 50 (08) :886-889

[23] PROPERTIES AND MODE OF ACTION OF THE POLYMYXINS [J].

NEWTON, BA .

BACTERIOLOGICAL REVIEWS, 1956, 20 (01) :14-27

[24] Formation of high-quality CdTe, CdSe, and CdS nanocrystals using CdO as precursor [J].

Peng, ZA ;

Peng, XG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (01) :183-184

[25] Blue shift of CdSe/ZnS nanocrystal-labels upon DNA-hybridization [J].

Riegler J. ;

Ditengou F. ;

Palme K. ;

Nann T. .

J. Nanobiotechnology, 2008, 6 (1)

[26] INTERACTION OF DIVALENT-CATIONS AND POLYMYXIN-B WITH LIPOPOLYSACCHARIDE [J].

SCHINDLER, M ;

OSBORN, MJ .

BIOCHEMISTRY, 1979, 18 (20) :4425-4430

[27] Photoactivation of quantum dot fluorescence following endocytosis [J].

Silver, J ;

Ou, W .

NANO LETTERS, 2005, 5 (07) :1445-1449

[28] Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay [J].

Song, JM ;

Vo-Dinh, T .

ANALYTICA CHIMICA ACTA, 2004, 507 (01) :115-121

[29] Colistin: An antimicrobial for the 21st century? [J].

Stein, A ;

Raoult, D .

CLINICAL INFECTIOUS DISEASES, 2002, 35 (07) :901-902

[30] Quantum dot biolabeling coupled with immunomagnetic separation for detection of Escherichia coli O157:H7 [J].

Su, XL ;

Li, YB .

ANALYTICAL CHEMISTRY, 2004, 76 (16) :4806-4810

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