Osmotic stress-induced genetic rearrangements in Escherichia coli H10407 detected by randomly amplified polymorphic DNA analysis

被引：12

作者：

Jolivet-Gougeon, A ^{[1
]}

David-Jobert, S

Tamanai-Shacoori, Z

Ménard, C

Cormier, M

机构：

[1] Univ Rennes 1, Lab Microbiol Pharmaceut, F-35000 Rennes, France

[2] Univ Laval, CHUQ, Ctr Rech Infectiol, Quebec City, PQ G1V 4G2, Canada

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 12期

关键词：

D O I：

10.1128/AEM.66.12.5484-5487.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Randomly amplified polymorphic DNA (RAPD) analysis is a DNA polymorphism assay commonly used for fingerprinting genomes, After optimizing the reaction conditions, samples of Escherichia coli H10407 DNA were assayed to determine the influence of osmotic and/or oligotrophic stress on variations in RAPD banding patterns. Genetic rearrangements or DNA topology variations could be detected as changes in agarose gel electrophoresis banding profiles. A new amplicon generated using DNA extracted from bacteria prestarved by an osmotic stress and resuscitated in rich medium was observed. Enrichment improved recovery of mutator cells and allowed them to be detected in samples, suggesting that DNA modifications, such as stress-induced alterations and supercoiling phenomena, should be taken into consideration before beginning RAPD analyses.

引用

页码：5484 / 5487

页数：4

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