Microarray analysis of peroxisome proliferator-activated receptor γ-induced changes in gene expression in macrophages

被引：46

作者：

Hodgkinson, CP ^{[1
]}

Ye, S ^{[1
]}

机构：

[1] Univ Southampton, Div Human Genet, Sch Med, Southampton, Hants, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 308卷 / 03期

关键词：

peroxisome proliferator-activated receptor-gamma; macrophages; gene expression; microarray analysis;

D O I：

10.1016/S0006-291X(03)01416-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We used a combination of expression microarray and Northern blot analyses to identify target genes for peroxisome proliferator-activated receptor (PPAR) gamma in RAW264.7 macrophages. PPARgamma natural ligand 15-deoxy-Delta(12,14) prostaglandin and synthetic ligands ciglitazone and rosiglitazone increased the expression of scavenger receptor CD36 and ATP-binding cassette transporter A1, as well as adipophilin (a lipid droplet coating protein involved in intracellular lipid storage and transport), calpain (a protease implicated in ABCA1 protein degradation), and ADAM8 (a disintegrin and metalloprotease protein involved in cell adhesion). These findings are relevant to understanding the effect of PPARgamma activation on gene expression and cognate pathways in macrophages. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：505 / 510

页数：6

共 27 条

[1] Pleiotropic actions of peroxisome proliferator-activated receptors in lipid metabolism and atherosclerosis [J].

Barbier, O ;

Torra, IP ;

Duguay, Y ;

Blanquart, C ;

Fruchart, JC ;

Glineur, C ;

Staels, B .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2002, 22 (05) :717-726

[2] Peroxisome proliferator-activated receptor-γ-independent inhibition of macrophage activation by the non-thiazolidinedione agonist L-796,449 -: Comparison with the effects of 15-deoxy-Δ12,14-prostaglandin J2 [J].

Castrillo, A ;

Mojena, M ;

Hortelano, S ;

Boscá, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (36) :34082-34088

[3] PPAR-γ dependent and independent effects on macrophage-gene expression in lipid metabolism and inflammation [J].

Chawla, A ;

Barak, Y ;

Nagy, L ;

Liao, D ;

Tontonoz, P ;

Evans, RM .

NATURE MEDICINE, 2001, 7 (01) :48-52

[4] PPAR-α and PPAR-γ activators induce cholesterol removal from human macrophage foam cells through stimulation of the ABCA1 pathway [J].

Chinetti, G ;

Lestavel, S ;

Bocher, V ;

Remaley, AT ;

Neve, B ;

Torra, IP ;

Teissier, E ;

Minnich, A ;

Jaye, M ;

Duverger, N ;

Brewer, HB ;

Fruchart, JC ;

Clavey, V ;

Staels, B .

NATURE MEDICINE, 2001, 7 (01) :53-58

[5] 15-DEOXY-DELTA(12,14)-PROSTAGLANDIN J(2) IS A LIGAND FOR THE ADIPOCYTE DETERMINATION FACTOR PPAR-GAMMA [J].

FORMAN, BM ;

TONTONOZ, P ;

CHEN, J ;

BRUN, RP ;

SPIEGELMAN, BM ;

EVANS, RM .

CELL, 1995, 83 (05) :803-812

[6] Target genes of peroxisome proliferator-activated receptor γ in colorectal cancer cells [J].

Gupta, RA ;

Brockman, JA ;

Sarraf, P ;

Willson, TM ;

DuBois, RN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (32) :29681-29687

[7] Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases [J].

Heid, HW ;

Moll, R ;

Schwetlick, I ;

Rackwitz, HR ;

Keenan, TW .

CELL AND TISSUE RESEARCH, 1998, 294 (02) :309-321

[8] CD156 transgenic mice [J].

Higuchi, Y ;

Yasui, A ;

Matsuura, K ;

Yamamoto, S .

PATHOBIOLOGY, 2002, 70 (01) :47-54

[9] PPAR-γ agonists inhibit production of monocyte inflammatory cytokines [J].

Jiang, CY ;

Ting, AT ;

Seed, B .

NATURE, 1998, 391 (6662) :82-86

[10] Inflammation in Atherosclerosis [J].

Libby, Peter .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2012, 32 (09) :2045-2051

← 1 2 3 →