Microarray analysis of peroxisome proliferator-activated receptor γ-induced changes in gene expression in macrophages

被引：46

作者：

Hodgkinson, CP ^{[1
]}

Ye, S ^{[1
]}

机构：

[1] Univ Southampton, Div Human Genet, Sch Med, Southampton, Hants, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 308卷 / 03期

关键词：

peroxisome proliferator-activated receptor-gamma; macrophages; gene expression; microarray analysis;

D O I：

10.1016/S0006-291X(03)01416-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We used a combination of expression microarray and Northern blot analyses to identify target genes for peroxisome proliferator-activated receptor (PPAR) gamma in RAW264.7 macrophages. PPARgamma natural ligand 15-deoxy-Delta(12,14) prostaglandin and synthetic ligands ciglitazone and rosiglitazone increased the expression of scavenger receptor CD36 and ATP-binding cassette transporter A1, as well as adipophilin (a lipid droplet coating protein involved in intracellular lipid storage and transport), calpain (a protease implicated in ABCA1 protein degradation), and ADAM8 (a disintegrin and metalloprotease protein involved in cell adhesion). These findings are relevant to understanding the effect of PPARgamma activation on gene expression and cognate pathways in macrophages. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：505 / 510

页数：6

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