Fed-batch culture of a metabolically engineered Escherichia coli strain designed for high-level succinate production and yield under aerobic conditions

被引:95
作者
Lin, H
Bennett, GN
San, KY
机构
[1] Rice Univ, Dept Bioengn, Houston, TX 77251 USA
[2] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77251 USA
[3] Rice Univ, Dept Chem Engn, Houston, TX 77251 USA
关键词
metabolic engineering; metabolic pathways; fed batch reactor; aerobic succinate production; metabolites;
D O I
10.1002/bit.20458
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An aerobic succinate production system developed by Lin et al. (Metab Eng, in press) is capable of achieving the maximum theoretical succinate yield of 1.0 mol/mol glucose for aerobic conditions. It also exhibits high succinate productivity. This succinate production system is a mutant E. coli strain with five pathways inactivated: Delta sdhAB, Delta(ackA-pta), Delta poxB, Delta icIR, and Delta ptsG. The mutant strain also overexpresses Sorghum vulgare pepc. This mutant strain is designated HL27659k(pKK313). Fed-batch reactor experiments were performed for the strain HL27659k(pKK313) under aerobic conditions to determine and demonstrate its capacity for high-level succinate production. Results showed that it could produce 58.3 g/l of succinate in 59 h under complete aerobic conditions. Throughout the entire fermentation the average succinate yield was 0.94 +/- 0.07 mol/mol glucose, the average productivity was 1.08 +/- 0.06 g/l-h, and the average specific productivity was 89.7 +/- 73.40 mg/g-h. Strain HL27659k (pKK313) is, thus, capable of large-scale succinate production under aerobic conditions. The results also showed that the aerobic succinate production system using the designed strain HL27659k(pKK313) is more practical than conventional anaerobic succinate production systems. It has remarkable potential for industrial-scale succinate production and process optimization. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:775 / 779
页数:5
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