Physical, mechanical and degradation properties, and Schwann cell affinity of cross-linked chitosan films

被引：62

作者：

Cao, WL ^{[1
]}

Cheng, MY ^{[1
]}

Ao, Q ^{[1
]}

Gong, YD ^{[1
]}

Zhao, NM ^{[1
]}

Zhang, XF ^{[1
]}

机构：

[1] Tsing Hua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 2005年 / 16卷 / 06期

关键词：

chitosan; peripheral nerve regeneration; cross-linking; mechanical and physical properties; biodegradability; Schwann cell;

D O I：

10.1163/1568562053992496

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Three kinds of cross-linked chitosan films were prepared with hexamethylene diisocyanate (HDI), epichlorohydrin (ECH) and glutaraldehyde (GA) as cross-linking agents, respectively. The physical and mechanical properties, biodegradability and Schwann cell affinity of the cross-linked films were investigated. A significant decrease in the degradation rate in lysozyme solution and a large change in the mechanical properties were observed compared with non-cross-linked chitosan films. The protein adsorption on chitosan films was determined by means of enzyme-linked immunosorbent assay (ELISA). In comparison with the non-cross-linked films, the chitosan films cross-linked with HDI showed a significant increase (up to 40-50%) in both fibronectin and laminin adsorption, while the protein adsorption on the other two kinds of cross-linked films was similar to that on non-crosslinked films. In addition, cell culture revealed that the HDI cross-linked chitosan films enhanced the spread and proliferation of Schwann cells while the other cross-linked films delayed the cell proliferation. These results suggest that HDI cross-linking of chitosan films provides a combination of physical properties, biodegradability and Schwann cell affinity suitable for peripheral nerve regeneration.

引用

页码：791 / 807

页数：17

共 32 条

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