Pausing of RNA Polymerase II Disrupts DNA-Specified Nucleosome Organization to Enable Precise Gene Regulation

被引：326

作者：

Gilchrist, Daniel A. ^{[1
]}

Dos Santos, Gilberto ^{[1
]}

Fargo, David C. ^{[2
]}

Xie, Bin ^{[5
]}

Gao, Yuan ^{[4
,5
]}

Li, Leping ^{[3
]}

Adelman, Karen ^{[1
]}

机构：

[1] NIEHS, Mol Carcinogenesis Lab, NIH, Res Triangle Pk, NC 27709 USA

[2] NIEHS, Lib Informat Serv, NIH, Res Triangle Pk, NC 27709 USA

[3] NIEHS, Biostat Branch, NIH, Res Triangle Pk, NC 27709 USA

[4] Johns Hopkins Univ, Inst Cell Engn, Neurogenerat & Stem Cell Biol Program, Baltimore, MD 21205 USA

[5] Johns Hopkins Univ, Lieber Inst Brain Dev, Div Genom Epigenom & Bioinformat, Baltimore, MD 21205 USA

来源：

CELL | 2010年 / 143卷 / 04期

关键词：

DROSOPHILA-MELANOGASTER; PROMOTER ARCHITECTURE; GAGA FACTOR; POL-II; P-TEFB; TRANSCRIPTION; ELONGATION; CHROMATIN; BINDING; NELF;

D O I：

10.1016/j.cell.2010.10.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metazoan transcription is controlled through either coordinated recruitment of transcription machinery to the gene promoter or regulated pausing of RNA polymerase II (Pol II) in early elongation. We report that a striking difference between genes that use these distinct regulatory strategies lies in the "default" chromatin architecture specified by their DNA sequences. Pol II pausing is prominent at highly regulated genes whose sequences inherently disfavor nucleosome formation within the gene but favor occlusion of the promoter by nucleosomes. In contrast, housekeeping genes that lack pronounced Pol II pausing show higher nucleosome occupancy downstream, but their promoters are deprived of nucleosomes regardless of polymerase binding. Our results indicate that a key role of paused Pol II is to compete with nucleosomes for occupancy of highly regulated promoters, thereby preventing the formation of repressive chromatin architecture

引用

页码：540 / 551

页数：12

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