Nuclear Scaffold Attachment Sites within ENCODE Regions Associate with Actively Transcribed Genes

被引：21

作者：

Keaton, Mignon A. ^{[1
]}

Taylor, Christopher M. ^{[2
]}

Layer, Ryan M. ^{[3
]}

Dutta, Anindya ^{[1
]}

机构：

[1] Univ Virginia, Dept Biochem & Mol Genet, Charlottesville, VA 22903 USA

[2] Univ New Orleans, Dept Comp Sci, New Orleans, LA 70148 USA

[3] Univ Virginia, Dept Comp Sci, Charlottesville, VA 22903 USA

来源：

PLOS ONE | 2011年 / 6卷 / 03期

基金：

美国国家卫生研究院;

关键词：

GENOME-TILING ARRAYS; DNA-REPLICATION; HISTONE MODIFICATIONS; MAMMALIAN-CELLS; PROTEIN MATRIX; CHROMATIN LOOP; HELA-CELLS; IDENTIFICATION; ORGANIZATION; CHROMOSOME;

D O I：

10.1371/journal.pone.0017912

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The human genome must be packaged and organized in a functional manner for the regulation of DNA replication and transcription. The nuclear scaffold/matrix, consisting of structural and functional nuclear proteins, remains after extraction of nuclei and anchors loops of DNA. In the search for cis-elements functioning as chromatin domain boundaries, we identified 453 nuclear scaffold attachment sites purified by lithium-3,5-iodosalicylate extraction of HeLa nuclei across 30 Mb of the human genome studied by the ENCODE pilot project. The scaffold attachment sites mapped predominately near expressed genes and localized near transcription start sites and the ends of genes but not to boundary elements. In addition, these regions were enriched for RNA polymerase II and transcription factor binding sites and were located in early replicating regions of the genome. We believe these sites correspond to genome-interactions mediated by transcription factors and transcriptional machinery immobilized on a nuclear substructure.

引用

页数：10