Inhibitory effects of the flavonoids isolated from Waltheria indica on the production of NO, TNF-α and IL-12 in activated macrophages

被引:51
作者
Rao, YK
Fang, SH
Tzeng, YM [1 ]
机构
[1] Chaoyang Univ Technol, Inst Biotechnol, Wufeng 413, Taiwan
[2] China Med Univ, Dept Microbiol, Sch Med, Taichung 400, Taiwan
关键词
Waltheria indica; flavonoid; nitric oxide; tumor necrosis factor-alpha; interleukin-12;
D O I
10.1248/bpb.28.912
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Three flavonoids were isolated from the whole plants of Waltheria indica and biological properties investigated. On the basis of their spectroscopic data, these compounds were identified as (-)-epicatechin, quercetin, and tiliroside. These flavonoids significantly and dose-dependently inhibited the production of the inflammatory mediator nitric oxide (NO), and the cytokines (tumor necrosis factor (TNF)-alpha and interleukin (IL)-12), in lipopolysaccharide (LPS) and interferon (IFN)-gamma activated murine peritoneal macrophages, without displaying cytotoxicity. The order of inhibitory activity was quercetin > tiliroside >(-)-epicatechin. Furthermore, peritoneal macrophages were pre-activated with LPS/IFN-gamma for 24 h, and the inhibitory effects of the above mentioned isolates on the production of NO were determined after a further 24 h, to address the possible mechanisms of their action. The present study supports the use of W. indica for the treatment of inflammatory diseases in traditional medicine.
引用
收藏
页码:912 / 915
页数:4
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