The use of PCR genotyping in the assessment of recrudescence or reinfection after antimalarial drug treatment

被引:151
作者
Snounou, G [1 ]
Beck, HP
机构
[1] Imperial Coll Sch Med, Dept Infect & Trop Med, Lister Unit, Northwick Pk Hosp, Harrow HA1 3UJ, Middx, England
[2] Swiss Trop Inst, CH-4002 Basel, Switzerland
来源
PARASITOLOGY TODAY | 1998年 / 14卷 / 11期
关键词
D O I
10.1016/S0169-4758(98)01340-4
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
In the past, assessment of drug efficacy against Plasmodium falciparum malaria has been performed by microscopy, screening for parasites in blood smears. However, in areas of high endemicity, reappearing parasites might be derived from new inoculations and could be classified falsely as treatment failures. Recently, a number of studies have used polymerase chain reaction (PCR) analysis of detectable parasites after drug administration to discriminate new injections from true recrudescence. The feasibility, high sensitivity and high resolution of this technique proves that if will be practical and highly valuable in studies on both drug resistance and vaccine efficacy as well as the testing of novel antimalarial drugs. In this article, Georges Snounou and Hans-Peter Beck discuss the uncertainties in the interpretation of data inherent to the technical limitations of the PCR technique, and the constraints imposed by the biology of the parasite. They suggest that although genotyping can provide strong evidence for differentiating between true recrudescence and reinfection, if must be interpreted with caution. They also propose strategies that might help minimize these uncertainties.
引用
收藏
页码:462 / 467
页数:6
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[1]   Evidence that recurrent Plasmodium falciparum infection is caused by recrudescence of resistant parasites [J].
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SULTAN, A ;
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WALLIKER, D .
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Steiger, S ;
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Weiss, N ;
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[6]   Different genetic characteristics of Plasmodium falciparum isolates collected during successive clinical malaria episodes in Senegalese children [J].
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Rogier, C ;
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Konate, L ;
Trape, JF ;
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[7]   PCR TYPING OF FIELD ISOLATES OF PLASMODIUM-FALCIPARUM [J].
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BONNEFOY, S ;
SKOURI, F ;
NTOUMI, F ;
MERCEREAUPUIJALON, O .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (04) :944-951
[8]   LONGITUDINAL-STUDY OF PLASMODIUM-FALCIPARUM POLYMORPHIC ANTIGENS IN A MALARIA-ENDEMIC POPULATION [J].
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GREENWOOD, BM ;
MCBRIDE, JS .
INFECTION AND IMMUNITY, 1992, 60 (03) :1122-1127
[9]   POPULATION-GENETICS OF PLASMODIUM-FALCIPARUM WITHIN A MALARIA HYPERENDEMIC AREA [J].
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MORRY, MJ ;
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