pGIL01, a linear tectiviral plasmid prophage originating from Bacillus thuringiensis serovar israelensis

被引:65
作者
Verheust, C
Jensen, G
Mahillon, J
机构
[1] Catholic Univ Louvain, B-1348 Louvain, Belgium
[2] Natl Inst Occupat Hlth, DK-2100 Copenhagen, Denmark
来源
MICROBIOLOGY-SGM | 2003年 / 149卷
关键词
D O I
10.1099/mic.0.26307-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacillus thuringiensis serovar israelensis harbours, in addition to several circular plasmids, a small linear molecule of about 15 kb. Sequence analysis of this molecule, named pGIL01, showed the presence of at least 30 ORFs, five of which displayed similarity with proteins involved in phage systems: a B-type family DNA polymerase, a LexA-like repressor, two potential muramidases and a DNA-packaging protein (distantly related to the P9 protein of the tectiviral phage PRD1). Experimental evidence confirmed that pGIL01 indeed corresponds to the linear prophage of a temperate phage. This bacteriophage, named GIL01, produces small turbid plaques and is sensitive to organic solvents, which suggests the presence of lipid components in its capsid. Experiments using proteases and exonucleases also revealed that proteins are linked to the genomes of both pGIL01 prophage and GIL01 phage at their 5' extremities. Altogether, these features are reminiscent of those of phages found in the Tectiviridae family, and more specifically of those of PRD1, a broad-host-range phage of Gram-negative bacteria. Dot-blot hybridization, PFGE, PCR and RFLP analyses also showed the presence of pGIL01 variants in the Bacillus cereus group.
引用
收藏
页码:2083 / 2092
页数:10
相关论文
共 48 条
  • [31] The aggregation-mediated conjugation system of Bacillus thuringiensis subsp. israelensis: Host range and kinetics of transfer
    Jensen, GB
    Andrup, L
    Wilcks, A
    Smidt, L
    Poulsen, OM
    [J]. CURRENT MICROBIOLOGY, 1996, 33 (04) : 228 - 236
  • [32] Kanda K, 1998, ACTA VIROL, V42, P315
  • [33] Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group
    Lechner, S
    Mayr, R
    Francis, KP
    Prüss, BM
    Kaplan, T
    Wiessner-Gunkel, E
    Stewartz, GSAB
    Scherer, S
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 : 1373 - 1382
  • [34] Léonard C, 1998, INFECT IMMUN, V66, P2163
  • [35] FINE MAPPING AND DNA-SEQUENCE OF REPLICATION FUNCTIONS OF BACILLUS-THURINGIENSIS PLASMID PTX14-3
    MADSEN, SM
    ANDRUP, L
    BOE, L
    [J]. PLASMID, 1993, 30 (02) : 119 - 130
  • [36] φ29 family of phages
    Meijer, WJJ
    Horcajadas, JA
    Salas, M
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (02) : 261 - +
  • [37] ASSEMBLY OF BACTERIOPHAGE-PRD1 - PARTICLE FORMATION WITH WILD-TYPE AND MUTANT VIRUSES
    MINDICH, L
    BAMFORD, D
    MCGRAW, T
    MACKENZIE, G
    [J]. JOURNAL OF VIROLOGY, 1982, 44 (03) : 1021 - 1030
  • [38] NAGY E, 1974, ACTA MICROBIOL HUNG, V21, P257
  • [39] Bacillus pseudomycoides sp. nov.
    Nakamura, LK
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 : 1031 - 1035
  • [40] Identification of two linear plasmids in the actinomycete Planobispora rosea
    Polo, S
    Guerini, O
    Sosio, M
    Dehò, G
    [J]. MICROBIOLOGY-UK, 1998, 144 : 2819 - 2825