pGIL01, a linear tectiviral plasmid prophage originating from Bacillus thuringiensis serovar israelensis

被引:65
作者
Verheust, C
Jensen, G
Mahillon, J
机构
[1] Catholic Univ Louvain, B-1348 Louvain, Belgium
[2] Natl Inst Occupat Hlth, DK-2100 Copenhagen, Denmark
来源
MICROBIOLOGY-SGM | 2003年 / 149卷
关键词
D O I
10.1099/mic.0.26307-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacillus thuringiensis serovar israelensis harbours, in addition to several circular plasmids, a small linear molecule of about 15 kb. Sequence analysis of this molecule, named pGIL01, showed the presence of at least 30 ORFs, five of which displayed similarity with proteins involved in phage systems: a B-type family DNA polymerase, a LexA-like repressor, two potential muramidases and a DNA-packaging protein (distantly related to the P9 protein of the tectiviral phage PRD1). Experimental evidence confirmed that pGIL01 indeed corresponds to the linear prophage of a temperate phage. This bacteriophage, named GIL01, produces small turbid plaques and is sensitive to organic solvents, which suggests the presence of lipid components in its capsid. Experiments using proteases and exonucleases also revealed that proteins are linked to the genomes of both pGIL01 prophage and GIL01 phage at their 5' extremities. Altogether, these features are reminiscent of those of phages found in the Tectiviridae family, and more specifically of those of PRD1, a broad-host-range phage of Gram-negative bacteria. Dot-blot hybridization, PFGE, PCR and RFLP analyses also showed the presence of pGIL01 variants in the Bacillus cereus group.
引用
收藏
页码:2083 / 2092
页数:10
相关论文
共 48 条
  • [21] Mechanism of DNA replication in eukaryotic cells: cellular host factors stimulating adenovirus DNA replication
    de Jong, RN
    van der Vliet, PC
    [J]. GENE, 1999, 236 (01) : 1 - 12
  • [22] Dubnau D., 2002, BACILLUS SUBTILIS IT, P453
  • [23] Glare TR., 2000, BACILLUS THURINGIENS
  • [24] A LARGE TRANSMISSIBLE PLASMID IS REQUIRED FOR CRYSTAL TOXIN PRODUCTION IN BACILLUS-THURINGIENSIS VARIETY ISRAELENSIS
    GONZALEZ, JM
    CARLTON, BC
    [J]. PLASMID, 1984, 11 (01) : 28 - 38
  • [25] Components of the RP4 conjugative transfer apparatus form an envelope structure bridging inner and cuter membranes of donor cells: Implications for related macromolecule transport systems
    Grahn, AM
    Haase, J
    Bamford, DH
    Lanka, E
    [J]. JOURNAL OF BACTERIOLOGY, 2000, 182 (06) : 1564 - 1574
  • [26] Sequential model of phage PRD1 DNA delivery: active involvement of the viral membrane
    Grahn, AM
    Daugelavicius, R
    Bamford, DH
    [J]. MOLECULAR MICROBIOLOGY, 2002, 46 (05) : 1199 - 1209
  • [27] Bacillus cereus and its food poisoning toxins
    Granum, PE
    Lund, T
    [J]. FEMS MICROBIOLOGY LETTERS, 1997, 157 (02) : 223 - 228
  • [28] Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli
    Heidrich, C
    Templin, MF
    Ursinus, A
    Merdanovic, M
    Berger, J
    Schwarz, H
    de Pedro, MA
    Höltje, JV
    [J]. MOLECULAR MICROBIOLOGY, 2001, 41 (01) : 167 - 178
  • [29] Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis -: One species on the basis of genetic evidence
    Helgason, E
    Okstad, OA
    Caugant, DA
    Johansen, HA
    Fouet, A
    Mock, M
    Hegna, I
    Kolsto, AB
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (06) : 2627 - 2630
  • [30] THE GENETIC-BASIS OF THE AGGREGATION SYSTEM IN BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS IS LOCATED ON THE LARGE CONJUGATIVE PLASMID PXO16
    JENSEN, GB
    WILCKS, A
    PETERSEN, SS
    DAMGAARD, J
    BAUM, JA
    ANDRUP, L
    [J]. JOURNAL OF BACTERIOLOGY, 1995, 177 (10) : 2914 - 2917